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dc.contributor.author
Crivello, Martin
dc.contributor.author
Bonaventura, Maria Marta
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Chamson-Reig, Astrid
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Arany, Edith
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Bettler, Bernhard
dc.contributor.author
Libertun, Carlos
dc.contributor.author
Lux, Victoria Adela R.
dc.date.available
2015-10-29T18:42:17Z
dc.date.issued
2013-03-26
dc.identifier.citation
Crivello, Martin; Bonaventura, Maria Marta; Chamson-Reig, Astrid; Arany, Edith; Bettler, Bernhard; et al.; Postnatal development of the endocrine pancreas in mice lacking functional GABAB receptors; American Physiological Society; American Journal of Physiology-Endocrinology and Metabolism; 304; 10; 26-3-2013; E1064-E1076
dc.identifier.issn
0193-1849
dc.identifier.uri
http://hdl.handle.net/11336/2573
dc.description.abstract
Adult mice lacking functional GABAB receptors (GABAB1KO) have glucose metabolism alterations. Since GABAB receptors (GABABRs) are expressed in progenitor cells, we evaluated islet development in GABAB1KO mice. Postnatal day 4 (PND4) and adult, male and female, GABAB1KO, and wild-type littermates (WT) were weighed and euthanized, and serum insulin and glucagon was measured. Pancreatic glucagon and insulin content were assessed, and pancreas insulin, glucagon, PCNA, and GAD65/67 were determined by immunohistochemistry. RNA from PND4 pancreata and adult isolated islets was obtained, and Ins1, Ins2, Gcg, Sst, Ppy, Nes, Pdx1, and Gad1 transcription levels were determined by quantitative PCR. The main results were as follows: 1) insulin content was increased in PND4 GABAB1KO females and in both sexes in adult GABAB1KOs; 2) GABAB1KO females had more clusters (<500 μm(2)) and less islets than WT females; 3) cluster proliferation was decreased at PND4 and increased in adult GABAB1KO mice; 4) increased β-area at the expense of the α-cell area was present in GABAB1KO islets; 5) Ins2, Sst, and Ppy transcription were decreased in PND4 GABAB1KO pancreata, adult GABAB1KO female islets showed increased Ins1, Ins2, and Sst expression, Pdx1 was increased in male and female GABAB1KO islets; and 6) GAD65/67 was increased in adult GABAB1KO pancreata. We demonstrate that several islet parameters are altered in GABAB1KO mice, further pinpointing the importance of GABABRs in islet physiology. Some changes persist from neonatal ages to adulthood (e.g., insulin content in GABAB1KO females), whereas other features are differentially regulated according to age (e.g., Ins2 was reduced in PND4, whereas it was upregulated in adult GABAB1KO females).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Physiological Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Gaba
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Receptores B
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Islote
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Diabetes
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Islet Development
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Hormones
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Transcription Factors
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Glucagon/Blood
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Insulin/Blood
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Bioquímica y Biología Molecular
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
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Otras Medicina Básica
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Postnatal development of the endocrine pancreas in mice lacking functional GABAB receptors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1522-1555
dc.journal.volume
304
dc.journal.number
10
dc.journal.pagination
E1064-E1076
dc.journal.pais
Estados Unidos de América
dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Crivello, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Bonaventura, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Chamson-Reig, Astrid. Lawson Health Research Institute, St. Joseph’s Health Care; Canadá;
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Fil: Arany, Edith. Lawson Health Research Institute, St. Joseph’s Health Care; Canadá;
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Fil: Bettler, Bernhard. University of Basel; Suiza;
dc.description.fil
Fil: Libertun, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.fil
Fil: Lux, Victoria Adela R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.journal.title
American Journal of Physiology-Endocrinology and Metabolism
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpendo.00569.2012
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1152/ajpendo.00569.2012
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://ajpendo.physiology.org/content/304/10/E1064.full#ref-list-1
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