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dc.contributor.author
Badel, Jorge L.
dc.contributor.author
Greenshields, David
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Piquerez, Sophie J. M.
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Rallapalli, Ganashyam
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Fabro, Georgina
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Ishaque, Naveed
dc.contributor.author
Jones, Jonathan D. G.
dc.date.available
2017-10-03T15:34:48Z
dc.date.issued
2013-07
dc.identifier.citation
Badel, Jorge L.; Greenshields, David; Piquerez, Sophie J. M. ; Rallapalli, Ganashyam; Fabro, Georgina; et al.; In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments; American Phytopathological Society; Molecular Plant-Microbe Interactions; 26; 7; 7-2013; 745-757
dc.identifier.issn
0894-0282
dc.identifier.uri
http://hdl.handle.net/11336/25727
dc.description.abstract
The genome of the pathogenic oomycete Hyaloperonospora arabidopsidis is predicted to encode at least 134 high-confidence effectors (HaRxL) carrying the RxLR motif implicated in their translocation into plant cells. However, only four avirulence genes (ATR1, ATR13, ATR5, and ATR39) have been isolated. This indicates that identification of HaRxL effectors based on avirulence is low throughput. We aimed at rapidly identifying H. arabidopsidis effectors that contribute to virulence by developing methods to detect and quantify multiple candidates in bacterial mixed infections using either Illumina sequencing or capillary electrophoresis. In these assays, referred to here as in planta effector competition assays, we estimate the contribution to virulence of individual effectors by calculating the abundance of each HaRxL in the bacterial population recovered from leaves 3 days after inoculation relative to abundance in the initial mixed inoculum. We identified HaRxL that enhance Pseudomonas syringae pv. tomato DC3000 growth in some but not all Arabidopsis accessions. Further analysis showed that HaRxLL464, HaRxL75, HaRxL22, HaRxLL441, and HaRxL89 suppress pathogen-associated molecular pattern-triggered immunity (PTI) and localize to different subcellular compartments in Nicotiana benthamiana, providing evidence for a multilayered suppression of PTI by pathogenic oomycetes and molecular probes for the dissection of PTI.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Phytopathological Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Effectors
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Hyaloperonospora
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Arabidopsis
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Pseudomonas
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-10-02T19:05:36Z
dc.journal.volume
26
dc.journal.number
7
dc.journal.pagination
745-757
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Badel, Jorge L.. John Innes Institute; Reino Unido
dc.description.fil
Fil: Greenshields, David. John Innes Institute; Reino Unido
dc.description.fil
Fil: Piquerez, Sophie J. M.. John Innes Institute; Reino Unido
dc.description.fil
Fil: Rallapalli, Ganashyam. John Innes Institute; Reino Unido
dc.description.fil
Fil: Fabro, Georgina. John Innes Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Ishaque, Naveed. John Innes Institute; Reino Unido
dc.description.fil
Fil: Jones, Jonathan D. G.. John Innes Institute; Reino Unido
dc.journal.title
Molecular Plant-Microbe Interactions
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1094/MPMI-06-12-0154-R
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/10.1094/MPMI-06-12-0154-R
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