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dc.contributor.author
del Carmen, Silvina Andrea
dc.contributor.author
Zurita Turk, Meritxell
dc.contributor.author
Alvarenga Lima, Fernanda
dc.contributor.author
Coelho Dos Santos, Janete
dc.contributor.author
Leclercq, Sophie Yvette
dc.contributor.author
Chatel, Jean-Marc
dc.contributor.author
Azevedo, Vasco
dc.contributor.author
de Moreno, Maria Alejandra
dc.contributor.author
Miyoshi, Anderson
dc.contributor.author
Leblanc, Jean Guy Joseph
dc.date.available
2015-10-26T18:21:11Z
dc.date.issued
2013-09
dc.identifier.citation
del Carmen, Silvina Andrea; Zurita Turk, Meritxell; Alvarenga Lima, Fernanda; Coelho Dos Santos, Janete; Leclercq, Sophie Yvette; et al.; A novel interleukin-10 DNA mucosal delivery system attenuates intestinal inflammation in a mouse model; Biolife Sas; European Journal Of Inflammation; 11; 3; 9-2013; 641-654
dc.identifier.issn
1721-727X
dc.identifier.uri
http://hdl.handle.net/11336/2568
dc.description.abstract
Inflammatory bowel diseases (IBD) describe a group of complex intestinal disorders characterized by inflammation in the gastrointestinal tract. Current treatments for IBD include the use of anti-inflammatory drugs; furthermore, recombinant lactic acid bacteria have been used as a therapeutic vehicle for anti-inflammatory agents in IBD models. Interleukin-10 (IL-10) is one of the most important anti-inflammatory cytokines; however, its oral administration is limited because it is quickly degraded in the gastrointestinal tract and systemic treatments have led to undesirable side effects. In this study, an engineered invasive strain of Lactococcus (L.) lactis producing Fibronectin Binding Protein A (FnBPA+), from Staphylococcus aureus capable of delivering, directly inside eukaryotic cells, an eukaryotic DNA expression vector containing the ORF coding for IL-10 of Mus musculus (pValac:il-10) was developed and its functionality was evaluated using in vitro and in vivo assays. Functionality of the plasmid and the invasive strain was demonstrated by transfection and invasiveness assays using cell cultures and in vivo in mice by fluorescence microscopy. TNBS inoculated mice that received this novel strain showed lower damage scores in their large intestines (at both macroscopic and microscopic levels), lower microbial translocation to liver, and increased anti-inflammatory/pro-inflammatory cytokine ratios compared to mice that received L. lactis FnBPA+ without the pValac:il-10 plasmid. The effectiveness was demonstrated of this novel DNA delivery therapeutic strategy in the prevention of inflammation using a murine model of colitis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Biolife Sas
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
CROHN'S DISEASE
dc.subject
DNA DELIVERY SYSTEM
dc.subject
INTERLEUKIN-10
dc.subject
LACTOCOCCUS LACTIS
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Biotecnología relacionada con la Salud
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Biotecnología de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
A novel interleukin-10 DNA mucosal delivery system attenuates intestinal inflammation in a mouse model
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
11
dc.journal.number
3
dc.journal.pagination
641-654
dc.journal.pais
Italia
dc.journal.ciudad
Silvi Marina
dc.description.fil
Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
dc.description.fil
Fil: Zurita Turk, Meritxell. Universidade Federal Do Minas Gerais; Brasil;
dc.description.fil
Fil: Alvarenga Lima, Fernanda. Universidade Federal Do Minas Gerais; Brasil;
dc.description.fil
Fil: Coelho Dos Santos, Janete. No especifíca;
dc.description.fil
Fil: Leclercq, Sophie Yvette. No especifíca;
dc.description.fil
Fil: Chatel, Jean-Marc. Institut National de la Recherche Agronomique; Francia;
dc.description.fil
Fil: Azevedo, Vasco. Universidade Federal Do Minas Gerais; Brasil;
dc.description.fil
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
dc.description.fil
Fil: Miyoshi, Anderson. Universidade Federal Do Minas Gerais; Brasil;
dc.description.fil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucuman. Facultad de Medicina. Departamento de Investigacion. Catedra de Metodologia de la Invest.cientifica; Argentina
dc.journal.title
European Journal Of Inflammation
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/1721727X1301100308
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/1721727X1301100308
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