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dc.contributor.author
Croce, Anna Cleta
dc.contributor.author
Garbelli, Anna
dc.contributor.author
Moyano, Andrea del Huerto

dc.contributor.author
Soldano, Sara
dc.contributor.author
Tejeda Guzmán, Carlos
dc.contributor.author
Missirlis, Fanis
dc.contributor.author
Scolari, Francesca
dc.date.available
2025-03-11T10:47:12Z
dc.date.issued
2023-12
dc.identifier.citation
Croce, Anna Cleta; Garbelli, Anna; Moyano, Andrea del Huerto; Soldano, Sara; Tejeda Guzmán, Carlos; et al.; Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 1; 12-2023; 1-19
dc.identifier.issn
1422-0067
dc.identifier.uri
http://hdl.handle.net/11336/255839
dc.description.abstract
Malpighian tubules (MTs) are arthropod excretory organs crucial for the osmoregulation, detoxification and excretion of xenobiotics and metabolic wastes, which include tryptophan degradation products along the kynurenine (KYN) pathway. Specifically, the toxic intermediate 3-hydroxy kynurenine (3-HK) is metabolized through transamination to xanthurenic acid or in the synthesis of ommochrome pigments. Early investigations in Drosophila larval fat bodies revealed an intracellular autofluorescence (AF) that depended on tryptophan administration. Subsequent observations documented AF changes in the MTs of Drosophila eye-color mutants genetically affecting the conversion of tryptophan to KYN or 3-HK and the intracellular availability of zinc ions. In the present study, the AF properties of the MTs in the Asian tiger mosquito, Aedes albopictus, were characterized in different stages of the insect’s life cycle, tryptophan-administered larvae and blood-fed adult females. Confocal imaging and microspectroscopy showed AF changes in the distribution of intracellular, brilliant granules and in the emission spectral shape and amplitude between the proximal and distal segments of MTs across the different samples. The findings suggest AF can serve as a promising marker for investigating the functional status of MTs in response to metabolic alterations, contributing to the use of MTs as a potential research model in biomedicine.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Molecular Diversity Preservation International

dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
mosquito developmental stages
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fluorescent granules
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dietary intake
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tryptophan
dc.subject.classification
Bioquímica y Biología Molecular

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Ciencias Biológicas

dc.subject.classification
CIENCIAS NATURALES Y EXACTAS

dc.title
Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2025-03-11T09:59:11Z
dc.journal.volume
25
dc.journal.number
1
dc.journal.pagination
1-19
dc.journal.pais
Suiza

dc.description.fil
Fil: Croce, Anna Cleta. Consiglio Nazionale delle Ricerche; Italia
dc.description.fil
Fil: Garbelli, Anna. Consiglio Nazionale delle Ricerche; Italia
dc.description.fil
Fil: Moyano, Andrea del Huerto. Consiglio Nazionale delle Ricerche; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Soldano, Sara. Universita degli Studi di Pavia; Italia
dc.description.fil
Fil: Tejeda Guzmán, Carlos. No especifíca;
dc.description.fil
Fil: Missirlis, Fanis. No especifíca;
dc.description.fil
Fil: Scolari, Francesca. Universita degli Studi di Pavia; Italia
dc.journal.title
International Journal of Molecular Sciences

dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/25/1/245
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/ijms25010245
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