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dc.contributor.author
Rojas, Paola Andrea
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dc.contributor.author
Benavides, Fernando
dc.contributor.author
Blando, Jorge
dc.contributor.author
Pérez, Carlos
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dc.contributor.author
Cardenas, Kim
dc.contributor.author
Richie, Ellen
dc.contributor.author
Knudsen, Erik S.
dc.contributor.author
Johnson, David G.
dc.contributor.author
Senderowicz, Adrian M.
dc.contributor.author
Rodriguez Puebla, Marcelo L.
dc.contributor.author
Conti, Claudio
dc.date.available
2017-10-02T15:47:39Z
dc.date.issued
2009-06
dc.identifier.citation
Rojas, Paola Andrea; Benavides, Fernando; Blando, Jorge; Pérez, Carlos; Cardenas, Kim; et al.; Enhanced skin carcinogenesis and lack of thymus hyperplasia in transgenic mice expressing human cyclin D1b (CCND1b); Wiley; Molecular Carcinogenesis.; 48; 6; 6-2009; 508-516
dc.identifier.issn
0899-1987
dc.identifier.uri
http://hdl.handle.net/11336/25564
dc.description.abstract
Cyclin D1b is an alternative transcript of the cyclin D1 gene (CCND1) expressed in human tumors. Its abundance is regulated by a single base pair polymorphism at the exon 4/intron 4 boundary (nucleotide 870). Epidemiological studies have shown a correlation between the presence of the G870A allele (that favors the splicing for cyclin D1b) with increased risk and less favorable outcome in several forms of cancer. More recently, it has been shown that, unlike cyclin D1a, the alternative transcript D1b by itself has the capacity to transform fibroblasts in vitro. In order to study the oncogenic potential of cyclin D1b, we developed transgenic mice expressing human cyclin D1b under the control of the bovine K5 promoter (K5D1b mice). Seven founders were obtained and none of them presented any significant phenotype or developed spontaneous tumors. Interestingly, K5D1b mice do not develop the fatal thymic hyperplasia, which is characteristic of the cyclin D1a transgenic mice (K5D1a). Susceptibility to skin carcinogenesis was tested in K5D1b mice using two-stage carcinogenesis protocols. In two independent experiments, K5D1b mice developed higher papilloma multiplicity as compared with wild-type littermates. However, when K5D1b mice were crossed with cyclin D1KO mice, the expression of cyclin D1b was unable to rescue the carcinogenesis-resistant phenotype of the cyclin D1 KO mice. To further explore the role of cyclin D1b in mouse models of carcinogenesis we carried out in silico analysis and in vitro experiments to evaluate the existence of a mouse homologous of the human cyclin D1b transcript. We were unable to find any evidence of an alternatively spliced transcript in mouse Ccnd1. These results show that human cyclin D1b has different biological functions than cyclin D1a and confirm its oncogenic properties.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cyclin D1
dc.subject
Skin Carcinogenesis
dc.subject
Dmba
dc.subject
Tpa
dc.subject.classification
Reumatología
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dc.subject.classification
Medicina Clínica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Enhanced skin carcinogenesis and lack of thymus hyperplasia in transgenic mice expressing human cyclin D1b (CCND1b)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-25T18:30:21Z
dc.identifier.eissn
1098-2744
dc.journal.volume
48
dc.journal.number
6
dc.journal.pagination
508-516
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Rojas, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. University of Texas; Estados Unidos
dc.description.fil
Fil: Benavides, Fernando. University of Texas; Estados Unidos
dc.description.fil
Fil: Blando, Jorge. University of Texas; Estados Unidos
dc.description.fil
Fil: Pérez, Carlos. University of Texas; Estados Unidos
dc.description.fil
Fil: Cardenas, Kim. University of Texas; Estados Unidos
dc.description.fil
Fil: Richie, Ellen. University of Texas; Estados Unidos
dc.description.fil
Fil: Knudsen, Erik S.. Thomas Jefferson University; Estados Unidos
dc.description.fil
Fil: Johnson, David G.. University of Texas; Estados Unidos
dc.description.fil
Fil: Senderowicz, Adrian M.. Department of Health and Human Services. Food and Drug Administration. Center for Drug Evaluation and Research; Estados Unidos
dc.description.fil
Fil: Rodriguez Puebla, Marcelo L.. University of North Carolina; Estados Unidos
dc.description.fil
Fil: Conti, Claudio. University of Texas; Estados Unidos
dc.journal.title
Molecular Carcinogenesis.
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/mc.20489/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/mc.20489
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/purl/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705965/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/18942117
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