Thermal Gelation of Proteins from Cajanus cajan Influenced by pH and Ionic Strength

Abstract

Cajanus cajan [pigeon pea (PP)] is an important legume crop for subsistence agriculture and its seeds are an alternative plant-based protein source. PP protein isolates (PPI) are able to form heat-induced gels that could be used for food applications. The aim of this work was to study the infuence of pH (2.1, 3.9, 6.3, and 8.3) and ionic strength (μ) (0.10 and 0.54) on thermal stability and thermal gelation of PPI obtained by alkaline extraction (pH 8.0) and isoelectric precipitation. Thermal stability of PPI changed with pH variation at low ionic strength (μ=0.10), decreasing this dependence with the increase of ionic strength (μ=0.54). At μ=0.10, gelation capacity of PPI was lower at pH 2.1 and pH 3.9. These gels presented a coarse network, which entails low WHC. At pH 6.3 and pH 8.3, gels showed a solid-like character with a compact and homogeneous matrix, with better WHC. At μ=0.54, gel formation was favoured at pH 2.1 and pH 3.9. G′ 20/G′ 95 ratio values and diferential solubility results suggest that hydrogen bonds and electrostatic interactions could play an important role in gel formation at pH 6.3 and pH 8.3.