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dc.contributor.author
Lopez Bergami, Pablo Roberto
dc.contributor.author
Ronai, Ze´ev
dc.date.available
2017-09-29T20:46:39Z
dc.date.issued
2008
dc.identifier.citation
Lopez Bergami, Pablo Roberto; Ronai, Ze´ev; Requirements for PKC-augmented JNK activation by MKK4/7; Elsevier; International Journal of Biochemistry and Cellular Biology; 40; 5; 2008; 1055-1064
dc.identifier.issn
1357-2725
dc.identifier.uri
http://hdl.handle.net/11336/25508
dc.description.abstract
The c-Jun N-terminal kinases (JNKs) are activated in response to stress, DNA damage, and cytokines by MKK4 and MKK7. We recently demonstrated that PKC can augment the degree of JNK activation by phosphorylating JNK, which requires the adaptor protein RACK1. Here we report on the conditions required for PKC-dependent JNK activation. In vitro kinase assays reveal that PKC phosphorylation of JNK is not sufficient for its activation but rather augments JNK activation by canonical JNK upstream kinases MKK4 or MKK7 alone or in combination. Further, to enhance JNK activity, PKC phosphorylation of JNK should precede its phosphorylation by MKK4/7. Inhibition of PKC phosphorylation of JNK affects both early and late phases of JNK activation following UV-irradiation and reduces the apoptotic response mediated by JNK. These data provide important insight into the requirements for PKC activation of JNK signaling.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Melanoma
dc.subject
Cell Line
dc.subject
Map Kinase 4
dc.subject
Map Kinase 7
dc.subject
Protein Kinace C
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.subject.classification
Patología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Requirements for PKC-augmented JNK activation by MKK4/7
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-25T18:30:18Z
dc.identifier.eissn
1878-5875
dc.journal.volume
40
dc.journal.number
5
dc.journal.pagination
1055-1064
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Lopez Bergami, Pablo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Sanford-burnham Medical Research Institute; Estados Unidos
dc.description.fil
Fil: Ronai, Ze´ev. Sanford-burnham Medical Research Institute; Estados Unidos
dc.journal.title
International Journal of Biochemistry and Cellular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1357272507003950
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.biocel.2007.11.011
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2327215/
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