Artículo
Gene expression changes throughout the life cycle allow a bacterial plant pathogen to persist in diverse environmental habitats
de Pedro Jové, Roger; Corral, Jordi; Rocafort, Mercedes; Puigvert, Marina; Azam, Fàtima Latif; Vandecaveye, Agustina Irene
; Macho, Alberto P.; Balsalobre, Carlos; Coll, Núria S.; Orellano, Elena Graciela
; Valls, Marc; Orellano, Elena Graciela



Fecha de publicación:
12/2023
Editorial:
Public Library of Science
Revista:
PLoS Pathogens
ISSN:
1553-7374
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Bacterial pathogens exhibit a remarkable ability to persist and thrive in diverse ecological niches. Understanding the mechanisms enabling their transition between habitats is crucial to control dissemination and potential disease outbreaks. Here, we use Ralstonia solanacearum, the causing agent of the bacterial wilt disease, as a model to investigate pathogen adaptation to water and soil, two environments that act as bacterial reservoirs, and compare this information with gene expression in planta. Gene expression in water resembled that observed during late xylem colonization, with an intriguing induction of the type 3 secretion system (T3SS). Alkaline pH and nutrient scarcity—conditions also encountered during late infection stages–were identified as the triggers for this T3SS induction. In the soil environment, R. solanacearum upregulated stress-responses and genes for the use of alternate carbon sources, such as phenylacetate catabolism and the glyoxylate cycle, and downregulated virulence-associated genes. We proved through gain- and loss-of-function experiments that genes associated with the oxidative stress response, such as the regulator OxyR and the catalase KatG, are key for bacterial survival in soil, as their deletion cause a decrease in culturability associated with a premature induction of the viable but non culturable state (VBNC). This work identifies essential factors necessary for R. solanacearum to complete its life cycle and is the first comprehensive gene expression analysis in all environments occupied by a bacterial plant pathogen, providing valuable insights into its biology and adaptation to unexplored habitats.
Palabras clave:
Ralstonia solanacearum
,
Bacterial wilt
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
de Pedro Jové, Roger; Corral, Jordi; Rocafort, Mercedes; Puigvert, Marina; Azam, Fàtima Latif; et al.; Gene expression changes throughout the life cycle allow a bacterial plant pathogen to persist in diverse environmental habitats; Public Library of Science; PLoS Pathogens; 19; 12; 12-2023; 1-26
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