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dc.contributor.author
Villarreal, Fernando Daniel
dc.contributor.author
Martin, María Victoria
dc.contributor.author
Colaneri, Alejandro Cesar
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Gonzalez Schain, Nahuel Damian
dc.contributor.author
Perales, Mariano
dc.contributor.author
Martin, Mariana Laura
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Lombardo, Maria Cristina
dc.contributor.author
Braun, Hans Peter
dc.contributor.author
Bartoli, Carlos Guillermo
dc.contributor.author
Zabaleta, Eduardo Julian
dc.date.available
2017-09-29T19:41:11Z
dc.date.issued
2009-07
dc.identifier.citation
Villarreal, Fernando Daniel; Martin, María Victoria; Colaneri, Alejandro Cesar; Gonzalez Schain, Nahuel Damian; Perales, Mariano; et al.; Ectopic expression of mitochondrial gamma carbonic anhydrase 2 causes male sterility by anther indehiscence; Springer; Plant Molecular Biology; 70; 4; 7-2009; 471-485
dc.identifier.issn
0167-4412
dc.identifier.uri
http://hdl.handle.net/11336/25496
dc.description.abstract
Plant mitochondria include gamma-type carbonic anhydrases (γCAs) of unknown function. In Arabidopsis, the γCAs form a gene family of five members which all are attached to the NADH dehydrogenase complex (complex I) of the respiratory chain. Here we report a functional analysis of gamma carbonic anhydrase 2 (CA2). The gene encoding CA2 is constitutively expressed in all plant organs investigated but it is ten fold induced in flowers, particularly in tapetal tissue. Ectopic expression of CA2 in Arabidopsis causes male sterility in transgenic plants. In normal anther development, secondary thickenings of the endothecial cell wall cause anthers to open upon dehydration. Histological analyses revealed that abnormal secondary thickening prevents anther opening in 35S::CA2 transgenic plants. CA2 abundance in transgenic plants is increased 2–3 fold compared to wild-type plants as revealed by Western blotting analyses. Moreover, abundance of other members of the CA family, termed CA3 and CAL2, is increased in transgenic plants. Oxygen uptake measurements revealed that respiration in transgenic plants is mainly based on NADH reduction by the alternative NADH dehydrogenases present in plant mitochondria. Furthermore, the formation of reactive oxygen species (ROS) is very low in transgenic plants. We propose that reduction in ROS inhibits H2O2 dependent lignin polymerization in CA2 over-expressing plants, thereby causing male sterility.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Gamma Carbonic Anhydrases
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Mitochondria
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Reactive Oxigen Species
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Anther
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Arabidopsis Thaliana
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Ectopic expression of mitochondrial gamma carbonic anhydrase 2 causes male sterility by anther indehiscence
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-24T18:54:26Z
dc.journal.volume
70
dc.journal.number
4
dc.journal.pagination
471-485
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Villarreal, Fernando Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Martin, María Victoria. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Colaneri, Alejandro Cesar. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Gonzalez Schain, Nahuel Damian. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Perales, Mariano. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Martin, Mariana Laura. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Lombardo, Maria Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Braun, Hans Peter. Universitat Hannover; Alemania
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Fil: Bartoli, Carlos Guillermo. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
dc.description.fil
Fil: Zabaleta, Eduardo Julian. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Plant Molecular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11103-009-9484-z
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11103-009-9484-z


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