Evento
Crosstalk between adipocytes and breast cancer cells: modulation of nuclear receptor coactivator 3 (rac3) in fat cells
Lira, María Cecilia
; Rosa, Francisco Damián
; Soares Machado, Mileni
; Palma, Alejandra Graciela
; Marino, Gabriela Inés
; Costas, Monica Alejandra
; Rubio, Maria Fernanda







Tipo del evento:
Reunión
Nombre del evento:
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Fecha del evento:
13/11/2019
Institución Organizadora:
Sociedad Argentina de Investigación Clínica;
Asociación Argentina de Farmacología Experimenta;
Sociedad Argentina de Biología;
Sociedad Argentina de Protozoología;
Asociación Argentina de Nanomedicinas;
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio;
Título de la revista:
Medicina (Buenos Aires)
Editorial:
Fundación Revista Medicina
ISSN:
1669-9106
Idioma:
Inglés
Clasificación temática:
Resumen
Adipocytes account for the largest proportion among the cells that comprise breast tissue. Although they are considered to be a critical cell type in the tumor microenvironment of breast cancer, there is still unclear the molecular mechanisms that control their behavior in this context. We have demonstrated that the transcriptional coactivator RAC3 decreases during adipogenesis and its high expression in adipose tissue adjacent to breast tumors correlate with different markers of tumoral progression in patient samples. Therefore, the aim of this work was to further study the role of this molecule in an in vitro model. Conditioned media (CM) were collected from non-tumoral or tumoral breast human cell lines with the following RAC3 mRNA levels: MCF10A: 1.55 ± 0.73, MDA-MB-231: 167.20 ± 6.91, T47D: 571.00 ± 12.59. 3T3-L1 derived-murine adipocytes were stimulated with CM or serum-free medium. RAC3 expression levels measured by qPCR resulted significantly higher in adipocytes stimulated with CM from high RAC3 levels-expressing T47D cells (9.25 ± 1.02) compared to the other conditions: basal (1.00 ± 0.00), MCF10A (2.33 ± 0.96), MDA-MB-231 (2.80 ± 1.19) (p<0.05). As RAC3 is a NF-kB coactivator we studied by immunofluorescence the presence of its phosphorylated subunit p65. We observed a greater fluorescence intensity in nucleus when adipocytes were stimulated with CM from tumoral cell lines (MDA-MB-231 or T47D) compared to the other conditions. Even more, since TNF is a NF-kB target gene, we evaluated by dot plot its levels in CM from adipocytes post-stimulation. Adipocytes secreted more TNF after being stimulated with CM from T47D (1.41 ± 0.03) compared to all other conditions: basal (1.00 ± 0.00), MCF10A (1.08 ± 0.11), MDA-MB-231 (1.02 ± 0.04) (p<0.05). The values were relativized to basal condition and results are shown as the mean ± SEM. These results validate our previous findings and suggest RAC3 as a molecular key to understand changes in the adipokines pattern in the tumor context.
Palabras clave:
BREAST CANCER
,
RAC3
,
ADIPOCYTES
,
TNF
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Eventos de INST.DE INVEST.MEDICAS
Eventos de INST.DE INVEST.MEDICAS
Citación
Crosstalk between adipocytes and breast cancer cells: modulation of nuclear receptor coactivator 3 (rac3) in fat cells; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 129-129
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