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dc.contributor.author
Yannarelli, Gustavo Gabriel
dc.contributor.author
Dayan, Victor
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Pacienza, Natalia Alejandra
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Lee, Chyan Jang
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Medin, Jeffrey
dc.contributor.author
Keating, Armand
dc.date.available
2017-09-29T16:24:02Z
dc.date.issued
2013-09
dc.identifier.citation
Yannarelli, Gustavo Gabriel; Dayan, Victor ; Pacienza, Natalia Alejandra; Lee, Chyan Jang ; Medin, Jeffrey ; et al.; Human umbilical cord perivascular cells exhibit enhanced cardiomyocyte reprogramming and cardiac function after experimental acute myocardial infarction; Sage Publications; Cell Transplantation; 22; 9; 9-2013; 1651-1666
dc.identifier.issn
0963-6897
dc.identifier.uri
http://hdl.handle.net/11336/25425
dc.description.abstract
We were interested in evaluating the ability of the mesenchymal stromal cell (MSC) population, human umbilical cord perivascular cells (HUCPVCs), to undergo cardiomyocyte reprogramming in an established co-culture system with rat embryonic cardiomyocytes. Results were compared with human bone marrow-derived (BM) MSCs. The transcription factors GATA4 and Mef2c were expressed in HUCPVCs but not BM-MSCs at baseline, and at 7 days increased 7.6 and 3.5-fold respectively, compared with BM-MSCs. Although cardiac-specific gene expression increased in both cell types in co-culture, up-regulation was more significant in HUCPVCs, consistent with Mef2c-GATA4 synergism. Using a lentivector with eGFP transcribed from the a-myosin heavy chain ( a-MHC) promoter, we found that cardiac gene expression was greater in HUCPVCs than BM-MSCs after 14d co-culture (52±17% vs 29±6%, respectively). A higher frequency of HUCPVCs expressed a-MHC protein compared with BM-MSCs (11.6±0.9% vs 5.3±0.3%) however, both cell types retained MSC-associated determinants. We also assessed the ability of the MSC types to mediate cardiac regeneration in a NOD/SCID(gnull) mouse model of acute myocardial infarction (AMI). Fourteen days after AMI, cardiac function was significantly better in celltreated mice compared with control animals and HUCPVCs exhibited greater improvement. Although human cells persisted in the infarct area, the frequency of a-MHC expression was low. Our results indicate that HUCPVCs exhibit a greater degree of cardiomyocyte reprogramming but that differentiation for both cell types is partial. We conclude that HUCPVCs may be preferable to BM-MSCs in the cell therapy of AMI.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sage Publications
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
Cardiomyocyte Reprogramming
dc.subject
Mesenchymal Stromal Cell
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Human Umbilical Cord Perivascular Cells
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Cell Differentiation
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Cell Therapy
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Ética relacionada con Biotecnología Médica
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Biotecnología de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Human umbilical cord perivascular cells exhibit enhanced cardiomyocyte reprogramming and cardiac function after experimental acute myocardial infarction
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-28T18:13:41Z
dc.journal.volume
22
dc.journal.number
9
dc.journal.pagination
1651-1666
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Thousand Oaks
dc.description.fil
Fil: Yannarelli, Gustavo Gabriel. University Health Network. Prince Margaret Hospital. Cell Therapy Program; Canadá
dc.description.fil
Fil: Dayan, Victor. University Health Network. Prince Margaret Hospital. Cell Therapy Program; Canadá
dc.description.fil
Fil: Pacienza, Natalia Alejandra. University Health Network; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Lee, Chyan Jang. University Health Network; Canadá
dc.description.fil
Fil: Medin, Jeffrey. University Health Network; Canadá
dc.description.fil
Fil: Keating, Armand. University Health Network. Prince Margaret Hospital. Cell Therapy Program; Canadá. University of Toronto; Canadá
dc.journal.title
Cell Transplantation
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3727/096368912X657675
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.3727/096368912X657675