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dc.contributor.author
Tiraboschi, Georgina

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Isaac, Paula

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Breser, Maria Laura

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Angiolini, Virginia Andrea

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Rodriguez Berdini, Lucía
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Porporatto, Carina

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Bohl, Luciana Paola

dc.date.available
2025-02-12T14:25:53Z
dc.date.issued
2024-03
dc.identifier.citation
Tiraboschi, Georgina; Isaac, Paula; Breser, Maria Laura; Angiolini, Virginia Andrea; Rodriguez Berdini, Lucía; et al.; 1,25 dihydroxyvitamin D3-mediated effects on bovine innate immunity and on biofilm-forming Staphylococcus spp. isolated from cattle with mastitis; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 240; 3-2024; 1-47
dc.identifier.issn
0960-0760
dc.identifier.uri
http://hdl.handle.net/11336/254160
dc.description.abstract
Mastitis is one the most widespread and serious diseases in dairy cattle. Recurrent and chronic infections are often attributable to certain pathogenicity mechanisms in mastitis-causing pathogens such as Staphylococcus spp. These include growing in biofilm and invading cells, both of which make it possible to resist or evade antimicrobial therapies and the host’s immune system. This study tested the effects of active vitamin D3 (i.e., calcitriol or 1,25-dihydroxyvitamin D3) on the internalization and phagocytosis of biofilm-forming Staphylococcus spp. isolated from animals with mastitis. Two established bovine cell lines were used: MAC-T (mammary epithelial cells) and BoMac (macrophages). Calcitriol (0-200 nM) did not affect the viability of MAC-T cells nor that of BoMac cells after 24 and 72 h. Concentrations of 0-100 mM for 24 h upregulated the expression of 24-hydroxylase in MAC-T cells, but did not alter that of VDR. Pre-treatment of the cells with calcitriol for 24 h decreased the internalization of S. aureus V329 into MAC-T cells (0-100 nM), and stimulated the phagocytosis of the same strain and of S. xylosus 4913 (0-10 nM). Calcitriol and two conditioned media, obtained by treating the cells with 25-200 nM of the metabolite for 24 h, were also assessed in terms of their antimicrobial and antibiofilm activity. Neither calcitriol by itself nor the conditioned media affected staphylococcal growth or biofilm formation (0-200 nM for 12 and 24 h, respectively). In contrast, the conditioned media (0-100 nM for 24 h) decreased the biomass of preformed non-aureus staphylococcal biofilms and killed the bacteria within them, without affecting metabolic activity. These effects may be mediated by reactive oxygen species and proteins with antimicrobial and/or antibiofilm activity. In short, calcitriol could make pathogens more accessible to antimicrobial therapies and enhance bacterial clearance by professional phagocytes. Moreover, it may modulate the host’s endogenous defenses in the bovine udder and help combat preformed non-aureus staphylococcal biofilms (S. chromogenes 40, S. xylosus 4913, and/or S. haemolyticus 6). The findings confirm calcitriol’s potential as an adjuvant to prevent and/or treat intramammary infections caused by Staphylococcus spp., which would in turn contribute to reducing antibiotic use on dairy farms.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd

dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
active vitamin D3
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internalization
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phagocytosis
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antibiofilm effects
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biofilm-forming mastitis pathogens
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Staphylococcus spp.
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active vitamin D3
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internalization
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Otras Ciencias Veterinarias

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Ciencias Veterinarias

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CIENCIAS AGRÍCOLAS

dc.title
1,25 dihydroxyvitamin D3-mediated effects on bovine innate immunity and on biofilm-forming Staphylococcus spp. isolated from cattle with mastitis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2025-02-11T14:47:32Z
dc.journal.volume
240
dc.journal.pagination
1-47
dc.journal.pais
Estados Unidos

dc.description.fil
Fil: Tiraboschi, Georgina. Universidad Nacional de Villa María. Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica - Universidad Nacional de Villa María. Instituto Multidisciplinario de Investigación y Transferencia Agroalimentaria y Biotecnológica; Argentina
dc.description.fil
Fil: Isaac, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
dc.description.fil
Fil: Breser, Maria Laura. Universidad Nacional de Villa María. Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
dc.description.fil
Fil: Angiolini, Virginia Andrea. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
dc.description.fil
Fil: Rodriguez Berdini, Lucía. Universidad Nacional de Villa María. Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas; Argentina
dc.description.fil
Fil: Porporatto, Carina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
dc.description.fil
Fil: Bohl, Luciana Paola. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
dc.journal.title
Journal of Steroid Biochemistry and Molecular Biology

dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0960076024000566
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jsbmb.2024.106508
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