Evaluation of toxicity of silver nanoparticles sintetized by biological mediators

Abstract

Biosensors have aroused great interest in genetically modified organisms (GMO) detection assays. In this regard, immunosensors presents high specificity due to the antigen-antibody interaction. In this work, New Zealand rabbits were injected with a synthetic peptide from the soybean Cp4-EPSPS protein, which provides glyphosate resistance, by injecting a synthetic peptide. The antiCp4-EPSPS antibodies production was evaluated by electrophoresis (SDS-PAGE) and an ELISA was developed in order to study their specificity. The ELISA showed that the polyclonal antibodies were specific to Cp4-EPSPS. In addition, the anti-Cp4-EPSPS were immobilized onto a gold disk electrode and the antigen-antibody interaction was evaluated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The CV showed that the current detected by the modified electrode decreased after incubation with Cp4-EPSPS peptide solution, whereas EIS increased after the incubation. These results suggest that the Cp4-EPSPS peptide was immobilized onto the electrode, due to the specific interaction with the anti-Cp4-EPSPS. On the other hand, these results show that this antigen-antibody interaction can be detected by electrochemical techniques, suggesting that the anti-Cp4-EPSPS produced can be used in CP4-EPSPS electrochemical immunosensors development.