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dc.contributor.author
Vera Candioti, Luciana
dc.contributor.author
Gil García, M. D.
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Martínez Galera. M.
dc.contributor.author
Goicoechea, Hector Casimiro
dc.date.available
2017-09-28T20:43:58Z
dc.date.issued
2008-12
dc.identifier.citation
Vera Candioti, Luciana; Gil García, M. D.; Martínez Galera. M.; Goicoechea, Hector Casimiro; Chemometric assisted solid-phase microextraction for the determination of anti-inflammatory and antiepileptic drugs in river water by liquid chromatography diode array detection; Elsevier Science; Journal Of Chromatography - A; 1211; 1-2; 12-2008; 22-32
dc.identifier.issn
0021-9673
dc.identifier.uri
http://hdl.handle.net/11336/25386
dc.description.abstract
In the present work, an analytical method for the simultaneous determination of seven non steroidal anti-inflammatory drugs (naproxen, ketoprofen, diclofenac, piroxicam, indomethacin, sulindac and diflunisal) and the anticonvulsant carbamazepine is reported. The method involves preconcentration and clean-up by solid-phase microextraction using polydimethylsiloxane/divinylbenzene fibers, followed by liquid chromatography with diode array detection analysis. Parameters that affect the efficiency of the solid-phase microextraction step such as soaking solvent, soaking period, desorption period, stirring rate, extraction time, sample pH, ionic strength, organic solvent and temperature were investigated using a Plackett–Burman screening design. Then, the factors presenting significant positive effects on the analytical response (soaking period, stirring rate, stirring time) were considered in a further central composite design to optimize the operational conditions for the solid phase microextraction procedure. Additionally, multiple response simultaneous optimization by using the desirability function was used to find the optimum experimental conditions for the on-line solid-phase microextraction of analytes in river water samples coupled to liquid chromatography and diode array detection. The best results were obtained using a soaking period of 5 min, stirring rate of 1400rpm and stirring time of 44 min. The use of solidphase microextraction technique avoided matrix effect and allowed to quantify the analytes in riverwater samples by using Milli-Q based calibration graphs. Recoveries ranging from 71.6% to 122.8% for all pharmaceuticals proved the accuracy of the proposedmethod in riverwater samples. Method detection limits were in the range of 0.5–3.0ugL-1 and limits of quantitation (LOQs) were between 1.0 and 4.0ugL-1 for pharmaceutical compounds in river water samples. The expanded uncertainty associated to the measurement of the concentration ranged between 8.5% and 29.0% for 20ugL-1 of each analyte and between 9.0% and 29.5% for the average of different concentration levels. The main source of uncertainty was the calibration step in both cases.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Pharmaceuticals
dc.subject
Experimental Design
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Solid-Phase Microextraction
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Liquid-Liquid Chromatography
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Chemometric assisted solid-phase microextraction for the determination of anti-inflammatory and antiepileptic drugs in river water by liquid chromatography diode array detection
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-25T18:21:27Z
dc.journal.volume
1211
dc.journal.number
1-2
dc.journal.pagination
22-32
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Vera Candioti, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Gil García, M. D.. Universidad de Almería; España
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Fil: Martínez Galera. M.. Universidad de Almería; España
dc.description.fil
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal Of Chromatography - A
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.chroma.2008.09.093
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0021967308016713
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