Mostrar el registro sencillo del ítem

dc.contributor.author
Albani, Clara Maria
dc.contributor.author
Cumino, Andrea Carina
dc.contributor.author
Elissondo, María Celina
dc.contributor.author
Denegri, Guillermo Maria
dc.date.available
2017-09-28T19:51:13Z
dc.date.issued
2013-07
dc.identifier.citation
Albani, Clara Maria; Cumino, Andrea Carina; Elissondo, María Celina; Denegri, Guillermo Maria; Development of a cell line from Echinococcus granulosus germinal layer; Elsevier Science; Acta Tropica; 128; 1; 7-2013; 124-129
dc.identifier.issn
0001-706X
dc.identifier.uri
http://hdl.handle.net/11336/25359
dc.description.abstract
In vitro culture of parasitic helminths provides an important tool to study cell regeneration and physiology, as well as for molecular biology and genetic engineering studies. In the present study, we established in vitro propagation of cells from Echinococcus granulosus germinal cyst layer. E. granulosus germinal cells grew beyond 100 passages and showed no signs of reduced proliferation capacity. Microscopic analysis revealed that cells grew both attached to the substrate and in suspension, forming three-dimensional structures like mammalian stem cell aggregates. Examination of the chromosome number of attached germinal cells showed a high degree of heteroploidy, suggesting the occurrence of transformation during culture. Monolayer cells survived cryopreservation and were able to proliferate after thawing. Based on the characteristics displayed by E. granulosus germinal cells, we establish a cell line from the E. granulosus germinal layer. Furthermore, we propose that this cell line could be useful for drug screening and for obtaining parasite material.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Parasite
dc.subject
Cestode
dc.subject
Echinococcus Granulosus
dc.subject
Germinal Cells
dc.subject
Cryopreservation
dc.subject
Cell Line
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Development of a cell line from Echinococcus granulosus germinal layer
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-27T15:36:42Z
dc.journal.volume
128
dc.journal.number
1
dc.journal.pagination
124-129
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Albani, Clara Maria. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Elissondo, María Celina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Denegri, Guillermo Maria. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Acta Tropica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.actatropica.2013.07.001
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0001706X13001812


Archivos asociados

Documento no disponible

Comunidades y colecciones

Mostrar el registro sencillo del ítem