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Artículo

Detection of minimally disseminated disease in the cerebrospinal fluid of children with high-risk retinoblastoma by reverse transcriptase-polymerase chain reaction for GD2 synthase mRNA

Laurent, Viviana EuniceIcon ; Sampor, Claudia; Solernou, Verónica; Rossi, Jorge; Gabri, Mariano RolandoIcon ; Eandi Eberle, Silvia; García de Dávila, María T.; Alonso, Daniel FernandoIcon ; Chantada, Guillermo LuisIcon
Fecha de publicación: 09/2013
Editorial: Elsevier
Revista: European Journal Of Cancer
ISSN: 0959-8049
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Reumatología

Resumen

Aim: To evaluate minimally disseminated disease (MDD) in cytologically negative cerebrospinal fluid (CSF) specimens of patients with high-risk retinoblastoma by the detection of the synthase of ganglioside GD2 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR). Methods: The CSF was evaluated in 26 patients with high risk for CSF relapse: 14 with postlaminar optic nerve invasion, five of them with tumour at the resection margin, five with massive choroidal invasion, three with overt orbital extension and four patients with systemic metastasis. Serial CSF examinations were repeated at different time intervals according to stage and in the event of suspected relapse. GD2 synthase mRNA was evaluated by RT and nested PCR at each procedure. Results: MDD was present at diagnosis in six cases (23%) and it was significantly associated to massive optic nerve involvement or history of glaucoma (p < 0.05). Three of the children with positive MDD had a CSF relapse. Thirteen patients had negative MDD at diagnosis and one had a CSF relapse. In seven children no ARN could be obtained for PCR analysis and two subsequently relapsed. The probability of CSF relapse was 0.50 (95% confidence interval (CI) 0.13–0.88) for children with MDD and 0.08 (95% CI 0.02–0.46) for those with negative RT-PCR examination of the CSF at diagnosis (p = 0.03). Conclusions: MDD in the CSF detected by RT-PCR for GD2-synthase mRNA occurred in 31.7% of evaluable high-risk children with retinoblastoma with no initial central nervous system (CNS) involvement. It was significantly associated to optic nerve involvement and glaucoma and increased risk of CSF relapse.
Palabras clave: Minimally Disseminated Disease , Cerebrospinal Fluid , Retinoblastoma , Gd2 Synthase , Metastasis
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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URI: http://hdl.handle.net/11336/25354
URL: http://www.sciencedirect.com/science/article/pii/S0959804913003559
DOI: http://dx.doi.org/ 10.1016/j.ejca.2013.04.021
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Laurent, Viviana Eunice; Sampor, Claudia; Solernou, Verónica; Rossi, Jorge; Gabri, Mariano Rolando; et al.; Detection of minimally disseminated disease in the cerebrospinal fluid of children with high-risk retinoblastoma by reverse transcriptase-polymerase chain reaction for GD2 synthase mRNA; Elsevier; European Journal Of Cancer; 49; 13; 9-2013; 2892-2899
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