Síntesis de ATP extracelular a partir de ADP y CTP, UTP y GTP en células Caco-2

Abstract

(A) The time course of eATP synthesis from eCTP (100 μM) and eADP (3 μM, 6 μM and 12 μM) in the extracellular medium of Caco-2 cells was quantified by luminometry. Cells were incubated with the reaction mix and eCTP and eADP were added at 10 min. Data are means of 3 to 5 independent experiments run in duplicate for each [ADP]. (B) Production of eATP after 30 minutes exposure of Caco-2 cells to 100 μM eCTP and 12 μM eADP. Experiments were run in the presence of 5 mM eUDP or in its absence. The [eATP] was measured under conditions similar to the experiments in (A). Results are expressed as [eATP] in μM/mg of protein, from 4 to 7 independent experiments run in duplicate. (C) and (D) The time course of eATP accumulation in the presence of eUTP ( 100 μM, 10 μM, 1 μM) or 100 μM eGTP (D). Data are the means from 4 independent experiments in the case of 100 μM eUTP, 3 in the case of 100 μM eGTP, and 2 independent experiments in the case of 10 μM or 1 μM eUTP.