Síntesis de ATP extracelular a partir de ADP en celulas Caco-2

Abstract

(A) The time course of [eATP] synthetized from exogenous eADP (6–48 μM) in the extracellular medium of intact Caco-2 cells was quantified by luminometry. The cells were incubated with the luciferin-luciferase reaction mix and the [eADP] indicated in the figure were added at the time indicated by the arrow. Data are means of at least 3 independent experiments run in duplicate for each [eADP]. (B) Effect of treatment with Ap5A (adenylate kinase inhibitor) on eATP synthesis from eADP in Caco-2 cells. The cells were treated or not (w/o treatment) with 10 μM Ap5A and the [eATP] at 30 minutes was measured by luminometry under similar conditions as experiments in (A).