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Artículo

Genome of Epinotia aporema granulovirus (EpapGV), a polyorganotropic fast killing betabaculovirus with a novel thymidylate kinase gene

Ferrelli, Maria LeticiaIcon ; Salvador, Ricardo; Biedma, Marina ElizabethIcon ; Berretta, Marcelo FacundoIcon ; Haase, SantiagoIcon ; Sciocco, Alicia Inés; Ghiringhelli, Pablo DanielIcon ; Romanowski, VictorIcon
Fecha de publicación: 10/2012
Editorial: BioMed Central
Revista: BMC Genomics
ISSN: 1471-2164
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biotecnología Agrícola y Biotecnología Alimentaria

Resumen

Background: Epinotia aporema (Lepidoptera: Tortricidae) is an important pest of legume crops in South America. Epinotia aporema granulovirus (EpapGV) is a baculovirus that causes a polyorganotropic infection in the host larva. Its high pathogenicity and host specificity make EpapGV an excellent candidate to be used as a biological control agent. Results: The genome of Epinotia aporema granulovirus (EpapGV) was sequenced and analyzed. Its circular double-stranded DNA genome is 119,082 bp in length and codes for 133 putative genes. It contains the 31 baculovirus core genes and a set of 19 genes that are GV exclusive. Seventeen ORFs were unique to EpapGV in comparison with other baculoviruses. Of these, 16 found no homologues in GenBank, and one encoded a thymidylate kinase. Analysis of nucleotide sequence repeats revealed the presence of 16 homologous regions (hrs) interspersed throughout the genome. Each hr was characterized by the presence of 1 to 3 clustered imperfect palindromes which are similar to previously described palindromes of tortricid-specific GVs. Also, one of the hrs (hr4) has flanking sequences suggestive of a putative non-hr ori. Interestingly, two more complex hrs were found in opposite loci, dividing the circular dsDNA genome in two halves. Gene synteny maps showed the great colinearity of sequenced GVs, being EpapGV the most dissimilar as it has a 20 kb-long gene block inversion. Phylogenetic study performed with 31 core genes of 58 baculoviral genomes suggests that EpapGV is the baculovirus isolate closest to the putative common ancestor of tortricid specific betabaculoviruses. Conclusions: This study, along with previous characterization of EpapGV infection, is useful for the better understanding of the pathology caused by this virus and its potential utilization as a bioinsecticide.
Palabras clave: Baculovirus , EpapGV , Epinotia aporema , Thymidylate kinase
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)
Identificadores
URI: http://hdl.handle.net/11336/252945
URL: https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-548
DOI: https://doi.org/10.1186/1471-2164-13-548
Colecciones
Articulos(IBBM)
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Ferrelli, Maria Leticia; Salvador, Ricardo; Biedma, Marina Elizabeth; Berretta, Marcelo Facundo; Haase, Santiago; et al.; Genome of Epinotia aporema granulovirus (EpapGV), a polyorganotropic fast killing betabaculovirus with a novel thymidylate kinase gene; BioMed Central; BMC Genomics; 13; 1; 10-2012; 1-14
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