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dc.contributor.author
Pontes, Daniela Santos
dc.contributor.author
Innocentin, Silvia
dc.contributor.author
del Carmen, Silvina Andrea
dc.contributor.author
Franco Almeida, Juliana
dc.contributor.author
Leblanc, Jean Guy Joseph
dc.contributor.author
de Moreno, Maria Alejandra
dc.contributor.author
Blugeon, Sébastien
dc.contributor.author
Cherbuy, Claire
dc.contributor.author
Lefèvre, François
dc.contributor.author
Azevedo, Vasco
dc.contributor.author
Miyoshi, Anderson
dc.contributor.author
Langella, Philippe
dc.contributor.author
Chatel, Jean-Marc
dc.date.available
2017-09-28T15:05:26Z
dc.date.issued
2012-09-27
dc.identifier.citation
Pontes, Daniela Santos; Innocentin, Silvia; del Carmen, Silvina Andrea; Franco Almeida, Juliana; Leblanc, Jean Guy Joseph; et al.; Production of fibronectin binding protein A at the surface of lactococcus lactis increases plasmid transfer In vitro and in vivo; Public Library of Science; Plos One; 7; 9; 27-9-2012; 1-6; e44892-e44892
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/25289
dc.description.abstract
Lactococci are noninvasive lactic acid bacteria frequently used as protein delivery vectors and, more recently, as DNA delivery vehicles. We previously showed that Lactococcus lactis (LL) expressing the Fibronectin-Binding Protein A of Staphylococcus aureus (LL-FnBPA+) showed higher internalization rates in vitro in Caco-2 cells than the native (wt) lactococci and were able to deliver a eukaryotic Green Fluorescent Protein (GFP) expression plasmid in 1% of human Caco-2 cells. Here, using the bovine beta-lactoglobulin (BLG), one of the major cow's milk allergen, and GFP we characterized the potential of LL-FnBPA+ as an in vivo DNA vaccine delivery vehicle. We first showed that the invasive strain LL-FnBPA+ carrying the plasmid pValac:BLG (LL-FnBPA+ BLG) was more invasive than LL-BLG and showed the same invasivity as LL-FnBPA+. Then we demonstrated that the Caco-2 cells, co-incubated with LL-FnBPA+ BLG produced up to 30 times more BLG than the Caco-2 cells co-incubated with the non invasive LL-BLG. Using two different gene reporters, BLG and GFP, and two different methods of detection, EIA and fluorescence microscopy, we showed in vivo that: i) in order to be effective, LL-FnBPA+ required a pre-coating with Fetal Calf Serum before oral administration; ii) plasmid transfer occurred in enterocytes without regard to the strains used (invasive or not); iii) the use of LL-FnBPA+ increased the number of mice producing BLG, but not the level of BLG produced. We thus confirmed the good potential of invasive recombinant lactic acid bacteria as DNA delivery vector in vivo.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Fibronectin-Binding Protein A
dc.subject
Lactococcus Lactis
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Bovine Beta-Lactoglobulin
dc.subject
Dna Delivery
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Production of fibronectin binding protein A at the surface of lactococcus lactis increases plasmid transfer In vitro and in vivo
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-28T15:16:51Z
dc.identifier.eissn
1932-6203
dc.journal.volume
7
dc.journal.number
9
dc.journal.pagination
1-6; e44892-e44892
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Pontes, Daniela Santos. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Innocentin, Silvia. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.description.fil
Fil: Franco Almeida, Juliana. Institut National de la Recherche Agronomique; Francia. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
dc.description.fil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.description.fil
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.description.fil
Fil: Blugeon, Sébastien. Institut National de la Recherche Agronomique; Francia. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
dc.description.fil
Fil: Cherbuy, Claire. Institut National de la Recherche Agronomique; Francia. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
dc.description.fil
Fil: Lefèvre, François. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Azevedo, Vasco. Universidade Federal Do Minas Gerais. Instituto de Cs.biologicas; Brasil
dc.description.fil
Fil: Miyoshi, Anderson. Universidade Federal Do Minas Gerais. Instituto de Cs.biologicas; Brasil
dc.description.fil
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
dc.description.fil
Fil: Chatel, Jean-Marc. Institut National de la Recherche Agronomique; Francia. Centre de Cooperation Internationale En Recherche Agronomique Pour Le Developperment; Francia
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0044892
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0044892
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