Metabolic rate of wild-caught and laboratory-raised Phyllotis xanthopygus in response to thermal acclimation

Name: Metabolic rate of wild-caught and laboratory-raised Phyllotis xanthopygus in response to thermal acclimation
License: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Keywords: ALTITUDINAL GRADIENT

Datos de investigación

Metabolic rate of wild-caught and laboratory-raised Phyllotis xanthopygus in response to thermal acclimation

Autores: Menéndez Sammartino, Josefina Icon

; Sassi, Paola Lorena Icon

; Ruperto, Emmanuel Fabián Icon

; Taraborelli, Paula Andrea Icon

Publicador: Consejo Nacional de Investigaciones Científicas y Técnicas

Fecha de depósito: 15/01/2025

Fecha de creación: 2016/2017

Clasificación temática:

Zoología, Ornitología, Entomología, Etología; Ecología

Resumen

The dataset contains resting metabolic rate measurements, using an open-flow respirometry system, across a range of ambient temperatures after acclimation to three thermal treatments of wild-caught (F0 generation) Phyllotis xanthopygus collected at different elevations in the Central Andes of Argentina and of their offspring born and raised under laboratory conditions (F1 generation). For each individual, body mass and sex are provided, as well as litter ID to identify the dam and siblings.

Métodos

Study site: The study was carried out at two adjacent reserves, Manzano Histórico Reserve and Manzano‐Portillo de Piuquenes Reserve, Mendoza Province, Argentina. There, we established three sampling sites along an altitudinal transect, running East–West, at 1700 (33°35'35.8''S ‐ 69°24'15.6''W), 2300 (33°36'48.4''S ‐ 69°28'42.7''W) and 3100 (33°36'37.9''S ‐69°32'14.5''W) m a.s.l., separated by a linear distance of 7 and 5 km respectively. Animal capture and experimental design: We collected 10 adult Phyllotis xanthopygus from each study site (six females and four males from the sites at 1700 and 3100 m a.s.l. each and five females and five males from the site at 2300 m a.s.l.) to conform the F0 generation group (total n = 30), using Sherman traps. Collection took place during the austral summer months of 2016-2017. Once in the laboratory, animals were housed individually in 30x30x40 cm cages, provided with chinchilla food pellets and water ad libitum, and kept for eight weeks under 12L:12D photoperiod and at 22 ± 2°C (standard laboratory conditions) for acclimation. Within the following 20 days after capture, we recorded the births of the collected females. Four females from the site at 1700 m a.s.l., three from 2300 m a.s.l. and four from 3100 m a.s.l. gave birth in the lab, giving a total of 11 litters. Pups were kept with the mother during the lactation period (30 days), after which they were housed individually. Once acclimation to laboratory conditions was over and females had weaned their litters – week 8 after capture – all F0 individuals were sequentially acclimated to three experimental temperatures – 16°C, 25°C, and 31°C – for four weeks before metabolic trials. Meanwhile, weaned pups were maintained under the standard laboratory conditions (22°C) until 3 months of age, time at which they reach maturity (Canals et al., 2009). Once they reached adulthood, two or three siblings from each litter were randomly chosen to obtain 10 individuals born to mothers captured at each altitudinal site (nine males and one female from 1700 m a.s.l., six males and four females from 2300 m a.s.l. and eight males and two females from 3100 m a.s.l.). These laboratory-reared adult animals, which conform the F1 generation group (total n = 30), were then sequentially acclimated to the three experimental temperatures – 16°C, 25°C and 31°C – for four weeks before metabolic trials. Metabolic trials: Metabolic rate was measured as the rate of oxygen consumption (VO2) using an open-flow respirometry system (Field Metabolic System; Sable Systems, Anderson, NV). Since the species is nocturnal (Kramer et al., 1999; Sassi et al., 2015), all measurements were performed between 8:00 and 19:00 hs. Each animal was weighed and put in a metabolic chamber that received air at a rate of 730 ml/min, previously passed through CO2 and H2O absorbents (sodalime and calcium sulphate, respectively). Excurrent air was sampled every 1 second by the FMS O2 analyzer (Sable Systems, Anderson, NV) and oxygen consumption was calculated following equation 10.1 from Lighton (2008) using EXPEDATA data acquisition software (Sable Systems, Anderson, NV). Metabolic measurements were continuously taken at ambient temperatures (Ta) of 10°C, 15C°, 20°C, 25°C, 28°C, 30°C, 32°C, 34°C, 36C° and 38°C for 30 minutes each, except for the first one which lasted 50 minutes for the animal to settle down (ramping design adapted from Zhao et al., 2014). The chamber was placed inside a Peltier-effect cabinet and Ta was set and held constant by a PELT-5 temperature controller (Sable Systems, Anderson, NV). Metabolic rate at each ambient temperature was calculated from the consecutively lowest most stable 5 minutes period.

Palabras clave: ALTITUDINAL GRADIENT, ENERGETICS, METABOLIC RATE, PHENOTYPIC PLASTICITY, SMALL MAMMAL, TEMPERATURE

Alcance geográfico

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Identificador del recurso

URI: http://hdl.handle.net/11336/252534

Colecciones

Datos de Investigación(IADIZA)
Datos de Investigación de INST. ARG DE INVEST. DE LAS ZONAS ARIDAS

Datos de Investigación(SEDE CENTRAL)
Datos de Investigación de SEDE CENTRAL

Citación

Menéndez Sammartino, Josefina; Sassi, Paola Lorena; Ruperto, Emmanuel Fabián; Taraborelli, Paula Andrea; (2025): Metabolic rate of wild-caught and laboratory-raised Phyllotis xanthopygus in response to thermal acclimation. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/252534

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