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dc.contributor.author
Monge, Maria Eugenia
dc.contributor.author
Martinefski, Manuela Romina
dc.contributor.author
Bollini, Mariela
dc.contributor.author
Pontel, Lucas Blas
dc.contributor.other
Papa, Salvatore
dc.contributor.other
Bubici, Concetta
dc.date.available
2025-01-10T11:25:41Z
dc.date.issued
2023
dc.identifier.citation
Monge, Maria Eugenia; Martinefski, Manuela Romina; Bollini, Mariela; Pontel, Lucas Blas; UHPLC-HRMS-Based Analysis of S-Hydroxymethyl-Glutathione, GSH, and GSSG in Human Cells; Springer Nature Switzerland AG; 2675; 2023; 117-132
dc.identifier.isbn
978-1-0716-3246-8
dc.identifier.uri
http://hdl.handle.net/11336/252224
dc.description.abstract
Glutathione (GSH) is one of the main antioxidant molecules present in cells. It harbors a thiol group responsible for sustaining cellular redox homeostasis. This moiety can react with cellular electrophiles such as formaldehyde yielding the compound S-hydroxymethyl-GSH (HSMGSH). HSMGSH is the substrate of the enzyme alcohol dehydrogenase 5 (ADH5) and thus a key intermediate in formaldehyde metabolism. In this work, we describe a method for the chemical synthesis of HSMGSH and a pipeline to identify this compound in complex cell extracts by means of ultra-high-performance liquid chromatography coupled to high-resolution spectrometry (UHPLC-HRMS). This method also allows determining GSH and oxidized disulfide (GSSG) in the same samples, thus providing broad information about formaldehyde-glutathione metabolism.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Nature Switzerland AG
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
GLUTHATHIONE
dc.subject
FORMALDEHYDE
dc.subject
S-HYDROXYMETHYL-GLUTHATHIONE
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ADH5
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OXIDIZED GLUTHATHIONE
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UHPLC-HRMS
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Química Analítica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
UHPLC-HRMS-Based Analysis of S-Hydroxymethyl-Glutathione, GSH, and GSSG in Human Cells
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/bookPart
dc.type
info:ar-repo/semantics/parte de libro
dc.date.updated
2024-11-27T10:06:38Z
dc.journal.volume
2675
dc.journal.pagination
117-132
dc.journal.pais
Suiza
dc.description.fil
Fil: Monge, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
dc.description.fil
Fil: Martinefski, Manuela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
dc.description.fil
Fil: Bollini, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
dc.description.fil
Fil: Pontel, Lucas Blas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1007/978-1-0716-3247-5_10
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007/978-1-0716-3247-5_10
dc.conicet.paginas
132
dc.source.titulo
Metabolic Reprogramming: Methods in Molecular Biology
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