Synthesis and characterization of fluorinated graphene oxide nanosheets derived from Lissachatina fulica snail mucus and their biomedical applications

Abstract

The modern nanomedicine incorporates the multimodal treatments into a single formulation,offering innovative cancer therapy options. Nanosheets function as carriers,altering the solubility, biodistribution, and effectiveness of medicinal compounds,resulting in more efficient cancer treatments and reduced side effects. The non-toxicnature of fluorinated graphene oxide (FGO) nanosheets and their potential applicationsin medication delivery, medical diagnostics, and biomedicine distinguish themfrom others. Leveraging the unique properties of Lissachatina fulica snail mucus(LfSM), FGO nanosheets were developed to reveal the novel characteristics. Consequently,LfSM was utilized to create non-toxic, environmentally friendly, and longlastingFGO nanosheets. Ultraviolet–visible (UV–vis) spectroscopy revealed a prominentabsorbance peak at 235 nm. The characterization of the synthesized FGOnanosheets involved X-ray diffraction (XRD), Fourier transform infrared spectroscopy(FTIR), Raman, scanning electron microscopy (SEM), X-ray photoelectron spectroscopy(XPS), high-resolution transmission electron microscopy (HR-TEM), and atomicforce microscopy (AFM) analyses. The antimicrobial activity data demonstrated abroad spectrum of antibacterial effects against Escherichia coli, Bacillus subtilis, Klebsiellapneumoniae, and Pseudomonas aeruginosa. The cytotoxicity efficacy of LfSM-FGOnanosheets against pancreatic cancer cell line (PANC1) showed promising results at low concentrations. The study suggests that FGO nanosheets made from LfSM couldserve as alternate factors for in biomedical applications in the future.