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dc.contributor.author
Collins, Sebastián Enrique  
dc.contributor.author
Briand, Laura Estefania  
dc.contributor.other
Wachs, Israel E.  
dc.contributor.other
Bañares, Miguel A.  
dc.date.available
2024-12-23T15:11:39Z  
dc.date.issued
2023  
dc.identifier.citation
Collins, Sebastián Enrique; Briand, Laura Estefania; Case study 2: Modulation excitation spectroscopy (MES); Springer International Publishin; 44; 2023; 991-1003  
dc.identifier.isbn
978-3031071249  
dc.identifier.uri
http://hdl.handle.net/11336/251154  
dc.description.abstract
In this case of study, we provide examples of the use of infrared spectroscopy in attenuated total reflection (ATR) to investigate enzymes at work. An optimized ATR flow-thought cell was developed, which allows performing modulation excitation spectroscopy with phase sensitive detection (MES-PSD) experiments in a controlled way. The reversible effect of solvents with different hydrophobicity on the secondary structure of a Thermomyces lanuginosus lipase (TTL) was demonstrated by an analysis of the Amide I band. Finally, the lipase B of Candida antarctica (CALB), active to the enantioselective esterification of ketoprofen with alcohols was studied. MES-PSD analysis allowed to univocally identifying signals belonging to the interaction between ketoprofen and the lipase, that is the acyl enzyme intermediate.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer International Publishin  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
MES-PSD  
dc.subject
ATR-FTIR  
dc.subject
LIPASES  
dc.subject
ACYL-ENZYME COMPLEX  
dc.subject.classification
Otras Ciencias Químicas  
dc.subject.classification
Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Case study 2: Modulation excitation spectroscopy (MES)  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/bookPart  
dc.type
info:ar-repo/semantics/parte de libro  
dc.date.updated
2024-11-22T12:25:36Z  
dc.journal.volume
44  
dc.journal.pagination
991-1003  
dc.journal.pais
Suiza  
dc.description.fil
Fil: Collins, Sebastián Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina  
dc.description.fil
Fil: Briand, Laura Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/chapter/10.1007/978-3-031-07125-6_44  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/978-3-031-07125-6_44  
dc.conicet.paginas
1929  
dc.source.titulo
Springer Handbook of Advanced Catalyst Characterization  

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