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dc.contributor.author
Garrido, Paula Melisa
dc.contributor.author
Martin, Mariana Laura
dc.contributor.author
Negri, Pedro
dc.contributor.author
Eguaras, Martin Javier
dc.date.available
2017-09-25T19:27:01Z
dc.date.issued
2013-02
dc.identifier.citation
Garrido, Paula Melisa; Martin, Mariana Laura; Negri, Pedro; Eguaras, Martin Javier; A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis ; Int Bee Research Assoc; Journal Of Apicultural Research; 52; 2; 2-2013; 67-68
dc.identifier.issn
0021-8839
dc.identifier.uri
http://hdl.handle.net/11336/25068
dc.description.abstract
The ectoparasitic mite Varroa destructor is a serious problem for beekeeping. It sucks haemolymph from different stages of the bees causing significant damage (see review by Rosenkranz et al., 2010). Haemolymph collection from honey bees is required to perform immunological, physiological, nutritional and other assays. In the literature there are many dissimilar methodologies to extract and store haemolymph. The electrophoretic profile and protein contents of V. destructor have been little studied, but it is of great interest to evaluate the changes experienced by this tissue in response to different treatments in the host and its parasite. The aim of this study is therefore to propose a standard procedure for haemolymph collection and storage, extending previous studies and sharing our working experience.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Int Bee Research Assoc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Apis Mellifera
dc.subject
Varroa Destructor
dc.subject
Haemolymph
dc.subject
Standardized Method
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
A standardized method to extract and store haemolymph from Apis mellifera and the ectoparasite Varroa destructor for protein analysis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-21T18:01:21Z
dc.journal.volume
52
dc.journal.number
2
dc.journal.pagination
67-68
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Martin, Mariana Laura. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Negri, Pedro. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal Of Apicultural Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3896/IBRA.1.52.2.13
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3896/IBRA.1.52.2.13


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