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Sarlo, Edgardo  
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Quintana, Silvina  
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Perezlindo, Tamara  
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Iglesias, Azucena Elizabeth  
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Iturburu, Fernando Gastón  
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Menone, Mirta Lujan  
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Eguaras, Martin Javier  
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Medici, Sandra Karina  
dc.date.available
2024-12-13T13:06:20Z  
dc.date.issued
2023  
dc.identifier.citation
Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees; 48th International Apicultural Congress; Santiago de Chile; Chile; 2023; 265-265  
dc.identifier.uri
http://hdl.handle.net/11336/250436  
dc.description.abstract
Agrochemicals, and particularly insecticides in sublethal doses, are widely recognized as being among the group of stressorsthat drastically reduce the fitness of insects. Particularly, Apis mellifera is known for its association with agriculture to sublethalcontact with a wide range of this group of stressors that trigger different types of stress, such as nutritional stress. Based onthis, the aim of this research is to determine if the level of total body proteins and the expression of the Vitellogenin gene inthree days emerged bees varies before the topical exposure to a sublethal dose (LD10) of the pesticides Imidacloprid.Bifenthrin and Chlorpyrifos or a combination of pairs of them. The bioassay was performed on a total of 30 A mellifera workersper treatment, which were contaminated with each pesticide or combination of them and after 24 hours were sacrificed withliquid nitrogen. Total protein content was obtained according to Bradford (1976) while Vitelogenin mRNA levels weredetermined by RT-qPCR. No significant differences were observed in the total protein content between the control group andthe individual treatment groups with bifenthrin and chlorpyrifos, however in the rest of the treatments the total protein contentwas significantly lower than the control. A significantly difference in mRNA levels was also found between individuals in theuntreated group and all of the treated groups. Within the treated groups, the mRNA expression in the group in contact withbifenthrin was significantly higher than among all other groups. It was concluded that the individuals that were in contact withthe agrochemicals studied presented a lower synthesis of vitellogenin mRNA, which may coincide with studies in other insectspecies. While it was postulated that this type of agrochemicals to a greater or lesser degree, depending on the familystimulates the corpora allata generating an increase in the juvenile hormone and therefore, in the case of bees, an inhibition ofthe synthesis of vitellogenin.  
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application/pdf  
dc.language.iso
eng  
dc.publisher
Conmark Tourism & Event Management Company  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Honey Bees  
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pesticides  
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protein  
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Vitellogenin  
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Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
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Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees  
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info:eu-repo/semantics/publishedVersion  
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info:eu-repo/semantics/conferenceObject  
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info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2024-01-23T14:53:33Z  
dc.journal.pagination
265-265  
dc.journal.pais
Chile  
dc.journal.ciudad
Santiado de Chile  
dc.description.fil
Fil: Sarlo, Edgardo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina  
dc.description.fil
Fil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina  
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Fil: Perezlindo, Tamara. Fares Taie Biotecnología; Argentina  
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Fil: Iglesias, Azucena Elizabeth. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina  
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Fil: Iturburu, Fernando Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina  
dc.description.fil
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina  
dc.description.fil
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina  
dc.description.fil
Fil: Medici, Sandra Karina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina  
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info:eu-repo/semantics/altIdentifier/url/https://apimondia2023.com/docs/abstract-book.pdf  
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dc.coverage
Internacional  
dc.type.subtype
Congreso  
dc.description.nombreEvento
48th International Apicultural Congress  
dc.date.evento
2023-09-04  
dc.description.ciudadEvento
Santiago de Chile  
dc.description.paisEvento
Chile  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Federación Internacional de Asociaciones de Apicultura  
dc.source.libro
Apimondia: 48th International Apicultural Congress. Sustainable Beekeeping, from the south of the world. Abstract Book  
dc.date.eventoHasta
2023-09-08  
dc.type
Congreso