Development of SRAP markers to evaluate the genetic variability in Brachypodium distachyon complex

Abstract

Brachypodium distachyon has been accepted as a model grass species for genetics andmolecular genomics in cereals since 2001. However, the genetic variability present inBrachypodium spp. continue being the aim of many research. Therefore, the aim of this workwas to analyze the genetic variability present in different Brachypodium spp. from differentcountries and to find different open reading frames (ORFs) among them by using sequencerelatedamplified polymorphism (SRAP) markers. In our study, a total of 12 Brachypodium spp.accessions were selected from different countries. By using SRAP markers, the genetic variabilitywas evaluated and some fragments were sequenced to obtain differential ORF informationamong genotypes. SRAP molecular markers revealed high variability among accessionsbeing USA the accession that showed the most variability at the DNA level. The analysis ofsequenced SRAP fragments from monomorphic and polymorphic fragments as well as thoseobtained from new primer combinations targeted coding regions into B. distachyon availablegenome. SRAP molecular marker showed not only to be an excellent tool for Brachypodiumvariability studies but also to provide additional information about differential amplification ofopen reading frames.