Mostrar el registro sencillo del ítem
dc.contributor.author
Mermet Joret, Noëmie
dc.contributor.author
Moreno, Andrea
dc.contributor.author
Zbela, Agnieszka
dc.contributor.author
Ellendersen, Bárður Eyjólfsson
dc.contributor.author
Krauth, Nathalie
dc.contributor.author
von Philipsborn, Anne
dc.contributor.author
Piriz, Joaquin
dc.contributor.author
Lin, John Y.
dc.contributor.author
Nabavi, Sadegh
dc.date.available
2024-12-09T14:03:23Z
dc.date.issued
2023-09
dc.identifier.citation
Mermet Joret, Noëmie; Moreno, Andrea; Zbela, Agnieszka; Ellendersen, Bárður Eyjólfsson; Krauth, Nathalie; et al.; Dual-color optical activation and suppression of neurons with high temporal precision; eLife Sciences Publications; Elife; 9-2023; 1-31
dc.identifier.issn
2050-084X
dc.identifier.uri
http://hdl.handle.net/11336/249842
dc.description.abstract
A fundamental challenge in the optogenetic toolbox is that all opsins, regardless of their excitation spectra, are activated by blue light. We hypothesized that pairing a red-shifted channelrhodopsin with a blue light-sensitive anion channel of appropriately matching kinetics shall render neurons responsive to a red but not blue light. To achieve this, we used a semi-rational mutagenesis strategy to optimize the kinetics and light spectrum of a chloride channelrhodopsin. By pairing optimized variants of blue light-sensitive anion channel ZipACR with vfChrimson, a fast red-shifted channelrhodopsin, we created a system in which red light derives precise and faithful action potentials of high frequencies, while blue light, through shunting inhibition, nullifies the effect of the red-shifted ChR2. Additionally, by a simple switch between red and blue lights, one can effectively excite or inhibit the activity of the same neurons.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
eLife Sciences Publications
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
OPTOGENETICS
dc.subject
NEURON
dc.subject
ZIPACR
dc.subject
CHRIMSON
dc.subject.classification
Neurociencias
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Dual-color optical activation and suppression of neurons with high temporal precision
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-11-22T13:09:21Z
dc.journal.pagination
1-31
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Mermet Joret, Noëmie. University Aarhus; Dinamarca
dc.description.fil
Fil: Moreno, Andrea. University Aarhus; Dinamarca
dc.description.fil
Fil: Zbela, Agnieszka. University Aarhus; Dinamarca. University of Tasmania; Australia
dc.description.fil
Fil: Ellendersen, Bárður Eyjólfsson. No especifíca;
dc.description.fil
Fil: Krauth, Nathalie. No especifíca;
dc.description.fil
Fil: von Philipsborn, Anne. No especifíca;
dc.description.fil
Fil: Piriz, Joaquin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
dc.description.fil
Fil: Lin, John Y.. No especifíca;
dc.description.fil
Fil: Nabavi, Sadegh. No especifíca;
dc.journal.title
Elife
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.7554/eLife.90327.1
Archivos asociados