Mostrar el registro sencillo del ítem
dc.contributor.author
Raimunda, Daniel Cesar
dc.contributor.author
Padilla Benavides, Teresita
dc.contributor.author
Vogt, Stefan
dc.contributor.author
Boutigny, Sylvain
dc.contributor.author
Tomkinson, Kaleigh N.
dc.contributor.author
Finney, Lydia A.
dc.contributor.author
Argüello, Jose M
dc.date.available
2017-09-22T17:46:35Z
dc.date.issued
2013-01
dc.identifier.citation
Raimunda, Daniel Cesar; Padilla Benavides, Teresita; Vogt, Stefan; Boutigny, Sylvain; Tomkinson, Kaleigh N.; et al.; Periplasmic response upon disruption of transmembrane Cu transport in Pseudomonas aeruginosa; Royal Society of Chemistry; Metallomics; 5; 2; 1-2013; 144-151
dc.identifier.issn
1756-5901
dc.identifier.uri
http://hdl.handle.net/11336/24942
dc.description.abstract
Pseudomonas aeruginosa, an opportunistic pathogen, has two transmembrane Cu+ transport ATPases, CopA1 and CopA2. Both proteins export cytoplasmic Cu+ into the periplasm and mutation of either gene leads to attenuation of virulence. CopA1 is required for maintaining cytoplasmic copper levels, while CopA2 provides copper for cytochrome c oxidase assembly. We hypothesized that transported Cu+ ions would be directed to their destination via specific periplasmic partners and disruption of transport should affect the periplasmic copper homeostasis. Supporting this, mutation of either ATPase gene led to large increments in periplasmic cuproprotein levels. Toward identifying the proteins participating in this cellular response the periplasmic metalloproteome was resolved in non-denaturing bidimensional gel electrophoresis, followed by X-ray fluorescence visualization and identification by mass-spectrometry. A single spot containing the electron shuttle protein azurin was responsible for the observed increments in cuproprotein contents. In agreement, lack of either Cu+ -ATPase induced an increase in azu transcription. This is associated with an increase in the expression of anr and rpoS oxidative stress response regulators, rather than cueR, a copper sensing regulator. We propose that azurin overexpression and accumulation in the periplasm is part of the cellular response to cytoplasmic oxidative stress in P. aeruginosa.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Royal Society of Chemistry
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Pib-Atpases
dc.subject
Copper Transport
dc.subject
Azurin
dc.subject
Periplasm
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Periplasmic response upon disruption of transmembrane Cu transport in Pseudomonas aeruginosa
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-21T19:05:10Z
dc.journal.volume
5
dc.journal.number
2
dc.journal.pagination
144-151
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Raimunda, Daniel Cesar. Worcester Polytechnic Institute; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Padilla Benavides, Teresita. Worcester Polytechnic Institute; Estados Unidos
dc.description.fil
Fil: Vogt, Stefan. Argonne National Laboratory; Estados Unidos
dc.description.fil
Fil: Boutigny, Sylvain. Worcester Polytechnic Institute; Estados Unidos
dc.description.fil
Fil: Tomkinson, Kaleigh N.. Worcester Polytechnic Institute; Estados Unidos
dc.description.fil
Fil: Finney, Lydia A.. Argonne National Laboratory; Estados Unidos
dc.description.fil
Fil: Argüello, Jose M. Worcester Polytechnic Institute; Estados Unidos
dc.journal.title
Metallomics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2013/MT/c2mt20191g#!divAbstract
Archivos asociados