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dc.contributor.author
Suarez, U.  
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Quevedo, M.R.  
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Selva, Juan Pablo  
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Carballo, José  
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Zappacosta, Diego Carlos  
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Echenique, Carmen Viviana  
dc.date.available
2024-12-04T13:15:07Z  
dc.date.issued
2023  
dc.identifier.citation
Exploring PLT gene family for insights into parthenogenesis regulation in Eragrostis curvula; IV International Congress on Apomixis; Rosario; Argentina; 2023; 47-47  
dc.identifier.uri
http://hdl.handle.net/11336/249406  
dc.description.abstract
Apomixis is an asexual reproduction regulated by three mechanisms that together generate viable seeds genetically identical to the mother. These mechanisms are, 1) apomeiosis (lack of female meiosis), 2)parthenogenesis (embryo development without egg cell fertilization) and 3) pseudogamy (single fertilization ofthe polar nuclei). Depending on the species, parthenogenesis was found regulated by different genes. In Taraxacum officinale, two unlinked dominant loci control diplospory (DIP) and fertilization independent development of an embryo from the egg cell (PAR). InPennisetum squamulatum and Cenchus ciliaris BABYBOOM (BBM)-like genes were identified as candidates for parthenogenesis. Ectopic expression of these genes using egg cell specific promoter in rice showed autonomous embryo development. In normal developmentBBM genes were found expressed during fertilization triggered by the pollen. BBM genes belong to the plethora(PLT) family of transcription factors characterized by two conserved APETALA2 (AP2) binding domains and abbm-1 domain with functional implications for somatic embryogenesis.Eragrostis curvula is a diplosporous apomictic grass used as model for the study of apomixis. Several genetic and epigenetic resources have beendeveloped for this grass; however, the molecular regulation of parthenogenesis was not characterized yet. Inorder to test if parthenogenesis is also regulated by BBM in E. curvula a genome wide identification of PLTfamily was conducted. In this way the E. curvula genome annotation was used to identify genes with AP2domains. Further phylogenetic analysis showed that the ten PLT genes present in rice are also present in E. curvula. PLT5 and PLT6, which are BBM3 and BBM1 respectively, were analyzed in detail to characterize theirfunction in E. curvula. Specific PCR primers designed for these genes showed promising results since theexpression patterns shows similarities with rice. Moreover, furthermore cloning and sequencing of PLT5 andPLT6 from genotypes contrasting in the reproductive mode showed structural differences both at the genomic and transcript levels. Finally, we are testing if the expression of these genes is triggered by the pollen and canled to the autonomous embryo development in E. curvula.  
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application/pdf  
dc.language.iso
eng  
dc.publisher
Universidad Nacional de Rosario  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
APOMIXIS  
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PARTHENOGENESIS  
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Biotecnología Agrícola y Biotecnología Alimentaria  
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Biotecnología Agropecuaria  
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CIENCIAS AGRÍCOLAS  
dc.title
Exploring PLT gene family for insights into parthenogenesis regulation in Eragrostis curvula  
dc.type
info:eu-repo/semantics/publishedVersion  
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info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2024-09-19T15:09:15Z  
dc.journal.pagination
47-47  
dc.journal.pais
Argentina  
dc.journal.ciudad
Rosario  
dc.description.fil
Fil: Suarez, U.. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Quevedo, M.R.. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Selva, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina  
dc.description.fil
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina  
dc.description.fil
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://apomixis2023.com.ar/wp-content/uploads/APOMIXIS-2023-book-of-abstracts.pdf  
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Autor  
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dc.coverage
Internacional  
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Congreso  
dc.description.nombreEvento
IV International Congress on Apomixis  
dc.date.evento
2023-12-03  
dc.description.ciudadEvento
Rosario  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario  
dc.source.libro
Book of abstracts of the IV International Congress on Apomixis  
dc.date.eventoHasta
2023-12-07  
dc.type
Congreso