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Artículo

Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. paratuberculosis

Ingratta, Giselle Gabriela; Stempler, Ana; Fernández, BárbaraIcon ; Colavecchia, Silvia Beatriz; Jolly, AnaIcon ; Minatel, Leonardo; Paolicchi, Fernando Alberto; Mundo, Silvia Leonor
Fecha de publicación: 05/2023
Editorial: Elsevier Science
Revista: Veterinary Immunology And Immunopathology
ISSN: 0165-2427
e-ISSN: 1873-2534
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Ciencias Veterinarias

Resumen

Mycobacterium avium subsp. paratuberculosis (MAP) is an important pathogen that causes granulomatous enteritisknown as Johne?s disease or paratuberculosis (PTB). In this study an experimental model of calves infected withArgentinean isolates of MAP for 180 days was used to provide more data of the early PTB stages. Calves werechallenged by oral route with MAP strain IS900-RFLPA (MA; n = 3), MAP strain IS900-RFLPC (MC; n = 2) or mockinfected (MI; n = 2), and response to infection was evaluated through peripheral cytokine expression, MAP tissuedistribution and histopathological early-stage findings. Specific and varied levels of IFN-γ were only detected at80 days post-infection in infected calves. These data indicate that specific IFN-γ is not a useful indicator for earlydetection of MAP infection in our calf model. At 110 days post-infection, TNF-α expression was higher than IL-10in 4 of the 5 infected animals and a significant decrease of TNF-α expression was detected in infected vs. noninfected calves. All calves challenged were identified as infected by mesenteric lymph node tissue culture andreal time IS900 PCR. In addition, for lymph nodes samples, the agreement between these techniques was almostperfect (κ = 0.86). Colonization of tissues and levels of tissue infection varied between individuals. Evidence ofearly MAP dissemination to extraintestinal tissues such as the liver was detected by culture in one animal (MAPstrain IS900-RFLPA). In both groups microgranulomatous lesions were observed predominantly in the lymph nodes,with giant cells present only in the MA group. In summary, the findings described herein may indicate that localMAP strains induced specific immune responses with particularities that could suggest differences in their biological behavior. Further studies should be carried out in order to obtain an in-depth understanding of the influence of MAP strains in host-pathogen interactions and the outcome of disease.
Palabras clave: CALVES , EXPERIMENTAL INFECTION , EARLY STAGES , HISTOPATHOLOGY , CYTOKINES , PARATUBERCULOSIS
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/249343
URL: https://www.sciencedirect.com/journal/veterinary-immunology-and-immunopathology
DOI: http://dx.doi.org/10.1016/j.vetimm.2023.110595
Colecciones
Articulos(INPA)
Articulos de UNIDAD EJECUTORA DE INVESTIGACIONES EN PRODUCCION ANIMAL
Citación
Ingratta, Giselle Gabriela; Stempler, Ana; Fernández, Bárbara; Colavecchia, Silvia Beatriz; Jolly, Ana; et al.; Early-stage findings in an experimental calf model infected with Argentinean isolates of Mycobacterium avium subsp. paratuberculosis; Elsevier Science; Veterinary Immunology And Immunopathology; 259; 5-2023; 1-9
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