Mostrar el registro sencillo del ítem
dc.contributor.author
Morales Urrea, Diego Alberto
dc.contributor.author
López Córdoba, Alex
dc.contributor.author
Contreras, Edgardo Martin
dc.date.available
2024-11-29T11:47:58Z
dc.date.issued
2023-08
dc.identifier.citation
Morales Urrea, Diego Alberto; López Córdoba, Alex; Contreras, Edgardo Martin; Inactivation kinetics of horseradish peroxidase (HRP) by hydrogen peroxide; Nature; Scientific Reports; 13; 1; 8-2023; 1-12
dc.identifier.issn
2045-2322
dc.identifier.uri
http://hdl.handle.net/11336/249003
dc.description.abstract
In recent years, the peroxidase enzymes have generated wide interest in several industrial processes, such as wastewater treatments, food processing, pharmaceuticals, and the production of fine chemicals. However, the low stability of the peroxidases in the presence of hydrogen peroxide (H2O2) has limited its commercial use. In the present work, the effect of H2O2 on the inactivation of horseradish peroxidase (HRP) was evaluated. Three states of HRP (E0, E2, and E3) were identified. While in the absence of H2O2, the resting state E0 was observed, in the presence of low and high concentrations of H2O2, E2,and E3 were found, respectively. The results showed that HRP catalyzed the H2O2decomposition, forming the species Ex, which was catalytically inactive. Results suggest that this loss of enzymatic activity is an intrinsic characteristic of the studied HRP. A model from a modified version of the Dunford mechanism of peroxidases was developed, which was validated against experimental data and findings reported by the literature.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
HRP
dc.subject
HYDROGEN PEROXIDE
dc.subject
DEACTIVATION
dc.subject
KINETIC MODELING
dc.subject.classification
Otras Ingeniería del Medio Ambiente
dc.subject.classification
Ingeniería del Medio Ambiente
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Inactivation kinetics of horseradish peroxidase (HRP) by hydrogen peroxide
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-11-26T14:19:01Z
dc.journal.volume
13
dc.journal.number
1
dc.journal.pagination
1-12
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Morales Urrea, Diego Alberto. Universidad Pedagógica y Tecnológica de Colombia; Colombia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
dc.description.fil
Fil: López Córdoba, Alex. Universidad Pedagógica y Tecnológica de Colombia; Colombia
dc.description.fil
Fil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
dc.journal.title
Scientific Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-023-39687-1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/s41598-023-39687-1
Archivos asociados