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dc.contributor.author Peralta, Andrea Verónica
dc.contributor.author Maroniche, Guillermo Andrés
dc.contributor.author Alfonso, Victoria
dc.contributor.author Molinari, Maria Paula
dc.contributor.author Taboga, Oscar Alberto
dc.date.available 2017-09-21T21:54:38Z
dc.date.issued 2013-04
dc.identifier.citation Peralta, Andrea Verónica; Maroniche, Guillermo Andrés; Alfonso, Victoria; Molinari, Maria Paula; Taboga, Oscar Alberto; VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display; Elsevier; Virus Research; 175; 1; 4-2013; 87-90
dc.identifier.issn 0168-1702
dc.identifier.uri http://hdl.handle.net/11336/24899
dc.description.abstract The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject Baculovirus display
dc.subject Display
dc.subject Biotecnología
dc.subject VP1
dc.subject Foot-and-mouth disease virus
dc.subject.classification Ética relacionada con Biotecnología Agrícola
dc.subject.classification Biotecnología Agropecuaria
dc.subject.classification CIENCIAS AGRÍCOLAS
dc.title VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-09-21T18:54:51Z
dc.journal.volume 175
dc.journal.number 1
dc.journal.pagination 87-90
dc.journal.pais Países Bajos
dc.journal.ciudad Ámsterdam
dc.description.fil Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Molinari, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.journal.title Virus Research
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168170213001123
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.virusres.2013.03.018


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)