Micropropagation of two varieties of vitis vinifera Cabernet Franc and Pinot Noir, comparison of physiological and production parameters

Abstract

Grapevine (Vitis vinifera) is considered one of the most important fruit crops in the world, and theCabernet Franc and Pinot Noir varieties have high commercial value. The aim of this study was tocompare the in vitro performance of both varieties from explant establishment to in vitro rooting,testing different hormone concentrations. It started from mother plants obtained in vitro, from whichuninodal cuttings were taken for micropropagation. Disinfection consisted of washing with 70%alcohol and 20% sodium hypochlorite, with sterile water rinses. Explants were placed in a laminarflow chamber under aseptic conditions in a sterile growth medium. They were grown in a culturechamber with 16 hours of light: 8 hours of darkness and a temperature of 25°C. Establishment,growth, rooting and contamination data were recorded for eight weeks. The culture media usedwere Experiment 1: 50% Murashige and Skoog (MS) [1] medium; Experiment 2: MS at 50% plusindoleacetic acid (IAA) at 0.01%. The data were statistically analyzed; Cabernet Franc and PinotNoir had similar production standards, high vigor, good in vitro growth and direct rooting. Bothvarieties performed better on the auxin enriched medium.