Artículo
Use of an enzyme-linked immunosorbent assay that utilizes the Tc13Tul antigen of Trypanosoma cruzi to monitor patients after treatment with benznidazole
Santamaría, Ana Lorena; de Rissio, Ana María; Riarte, Adelina; Garavaglia, Patricia Andrea; Bruballa, Andrea Cecilia
; Rodríguez, Marcelo Adrián; Irazu, Lucía Elvira; Ruiz, Andres Mariano
; Garcia, Gabriela Andrea
Fecha de publicación:
03/2013
Editorial:
Elsevier
Revista:
Diagnostic Microbiology And Infectious Diseases
ISSN:
0732-8893
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Tc13Tul antigen is expressed in the mammalian stages of Trypanosoma cruzi, the etiological agent of Chagas’ disease. Here, we designed and validated an enzyme-linked immunosorbent assay using the recombinant Tc13Tul (Tc13Tul-ELISA) and found that it had 82.5% sensitivity and 97.05% of specificity. To evaluate whether the decrease in antibodies against Tc13Tul may be used as an early marker of the effect of chemotherapy with benznidazole, sera from 30 T. cruzi-infected children were evaluated by Tc13Tul-ELISA before and after benznidazole treatment. While in Group A (6 months-4 years old, n = 16) the decrease of more than 30% of Tc13Tul-ELISA values showed a sensitivity similar to that of conventional serology (CS); in Group B, (5–12 years old, n = 14) the decrease of Tc13Tul-ELISA values was a better parameter than negativization of CS to monitor the impact of treatment. Therefore, the dosage of anti-Tc13Tul antibodies may be useful as a methodology complementary to CS to evaluate chagasic patients undergoing chemotherapy with benznidazole.
Palabras clave:
Chagas´ Disease
,
Chemotherapy
,
Diagnosis
,
Trans-Sialidase
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Santamaría, Ana Lorena; de Rissio, Ana María; Riarte, Adelina; Garavaglia, Patricia Andrea; Bruballa, Andrea Cecilia; et al.; Use of an enzyme-linked immunosorbent assay that utilizes the Tc13Tul antigen of Trypanosoma cruzi to monitor patients after treatment with benznidazole; Elsevier; Diagnostic Microbiology And Infectious Diseases; 76; 2; 3-2013; 197-205
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