Mostrar el registro sencillo del ítem

dc.contributor.author
Díaz, M.C.
dc.contributor.author
González, N.V.
dc.contributor.author
Zanuzzi, Carolina Natalia
dc.contributor.author
Najle, Roberto
dc.contributor.author
Barbeito, Claudio Gustavo
dc.date.available
2017-09-20T18:10:46Z
dc.date.issued
2017-01
dc.identifier.citation
Díaz, M.C.; González, N.V.; Zanuzzi, Carolina Natalia; Najle, Roberto; Barbeito, Claudio Gustavo; Lectin histochemistry for detecting cadmium-induced changes in the glycosylation pattern of rat placenta; Informa Healthcare; Biotechnic And Histochemistry; 92; 1; 1-2017; 36-45
dc.identifier.issn
1052-0295
dc.identifier.uri
http://hdl.handle.net/11336/24697
dc.description.abstract
Cadmium (Cd) is an industrial and environmental pollutant that produces toxic effects on gametogenesis, pre- and post-implantation embryos, and the placenta. Because the effects of acute Cd intoxication on the placenta are not well understood, we investigated changes in its glycosylated components in Cd treated dams at days 4, 7, 10 and 15 of gestation using lectin histochemistry. CdCl2 was administered to pregnant rats; control animals received sterile normal saline. Placentas were processed for DBA, Con A, SBA, PNA, UEA-I, RCA-I and WGA lectin histochemistry to evaluate changes in the carbohydrate pattern of the placenta that might modify cell interactions and contribute to embryonic alterations. Lectin binding was analyzed in the yolk sac; trophoblast giant cells; trophoblast I, II and III; spongiotrophoblast cells and endovascular trophoblast cells in the chorioallantoic placenta. Our lectin binding patterns showed that Cd caused alteration of SBA and DBA labeling of trophoblast-derived cells, which suggested increased expressions of α and β GalNAc. Cd also caused decreased UEA-1 binding affinity, which indicated fewer α-L-Fuc residues in placentas of Cd treated dams. The nonreactivity in trophoblast I of the control placentas incubated with Con-A contrasted with the labeling in placentas of experimental dams, which indicated increased expression of terminal α-D-Man, and α-D-Glc residues. We found that Cd altered the reactivity of placenta to several lectins, which indicated modification of the glycotype presented by the fetal component of the placenta. We report that Cd exerts a deleterious effect on the glycosylation pattern of the placenta.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Informa Healthcare
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cadmium
dc.subject
Carbohydrate Moieties
dc.subject
Glycoproteins
dc.subject
Lectin Histochemistry
dc.subject
Placenta
dc.subject
Rat
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Lectin histochemistry for detecting cadmium-induced changes in the glycosylation pattern of rat placenta
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-13T18:17:39Z
dc.journal.volume
92
dc.journal.number
1
dc.journal.pagination
36-45
dc.journal.pais
Reino Unido
dc.journal.ciudad
Baltimore
dc.description.fil
Fil: Díaz, M.C.. Universidad Nacional del Centro de Buenos Aires. Colegio de Veterinaria. Tandil; Argentina
dc.description.fil
Fil: González, N.V.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Najle, Roberto. Universidad Nacional del Centro de Buenos Aires. Colegio de Veterinaria. Tandil; Argentina
dc.description.fil
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.journal.title
Biotechnic And Histochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/10520295.2016.1185668
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.1080/10520295.2016.1185668


Archivos asociados

Documento no disponible

Comunidades y colecciones

Mostrar el registro sencillo del ítem