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dc.contributor.author Díaz, M.C.
dc.contributor.author González, N.V.
dc.contributor.author Zanuzzi, Carolina Natalia
dc.contributor.author Najle, Roberto
dc.contributor.author Barbeito, Claudio Gustavo
dc.date.available 2017-09-20T18:10:46Z
dc.date.issued 2017-01
dc.identifier.citation Díaz, M.C.; González, N.V.; Zanuzzi, Carolina Natalia; Najle, Roberto; Barbeito, Claudio Gustavo; Lectin histochemistry for detecting cadmium-induced changes in the glycosylation pattern of rat placenta; Informa Healthcare; Biotechnic And Histochemistry; 92; 1; 1-2017; 36-45
dc.identifier.issn 1052-0295
dc.identifier.uri http://hdl.handle.net/11336/24697
dc.description.abstract Cadmium (Cd) is an industrial and environmental pollutant that produces toxic effects on gametogenesis, pre- and post-implantation embryos, and the placenta. Because the effects of acute Cd intoxication on the placenta are not well understood, we investigated changes in its glycosylated components in Cd treated dams at days 4, 7, 10 and 15 of gestation using lectin histochemistry. CdCl2 was administered to pregnant rats; control animals received sterile normal saline. Placentas were processed for DBA, Con A, SBA, PNA, UEA-I, RCA-I and WGA lectin histochemistry to evaluate changes in the carbohydrate pattern of the placenta that might modify cell interactions and contribute to embryonic alterations. Lectin binding was analyzed in the yolk sac; trophoblast giant cells; trophoblast I, II and III; spongiotrophoblast cells and endovascular trophoblast cells in the chorioallantoic placenta. Our lectin binding patterns showed that Cd caused alteration of SBA and DBA labeling of trophoblast-derived cells, which suggested increased expressions of α and β GalNAc. Cd also caused decreased UEA-1 binding affinity, which indicated fewer α-L-Fuc residues in placentas of Cd treated dams. The nonreactivity in trophoblast I of the control placentas incubated with Con-A contrasted with the labeling in placentas of experimental dams, which indicated increased expression of terminal α-D-Man, and α-D-Glc residues. We found that Cd altered the reactivity of placenta to several lectins, which indicated modification of the glycotype presented by the fetal component of the placenta. We report that Cd exerts a deleterious effect on the glycosylation pattern of the placenta.
dc.format application/pdf
dc.language.iso eng
dc.publisher Informa Healthcare
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CADMIUM
dc.subject CARBOHYDRATE MOIETIES
dc.subject GLYCOPROTEINS
dc.subject LECTIN HISTOCHEMISTRY
dc.subject PLACENTA
dc.subject RAT
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Lectin histochemistry for detecting cadmium-induced changes in the glycosylation pattern of rat placenta
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-13T18:17:39Z
dc.journal.volume 92
dc.journal.number 1
dc.journal.pagination 36-45
dc.journal.pais Reino Unido
dc.journal.ciudad Baltimore
dc.description.fil Fil: Díaz, M.C.. Universidad Nacional del Centro de Buenos Aires. Colegio de Veterinaria. Tandil; Argentina
dc.description.fil Fil: González, N.V.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil Fil: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Najle, Roberto. Universidad Nacional del Centro de Buenos Aires. Colegio de Veterinaria. Tandil; Argentina
dc.description.fil Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.journal.title Biotechnic And Histochemistry
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/10520295.2016.1185668
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.1080/10520295.2016.1185668


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)