Mostrar el registro sencillo del ítem
dc.contributor.author
Naum Ongania, Gabriela
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Díaz Cortés, Víctor Manuel
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Blasi, Francesco
dc.contributor.author
Rivera Pomar, Rolando
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2017-09-19T19:53:33Z
dc.date.issued
2014-01
dc.identifier.citation
Naum Ongania, Gabriela; Díaz Cortés, Víctor Manuel; Blasi, Francesco; Rivera Pomar, Rolando; Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?; Landes Biosciences; Transcription; 4; 5; 1-2014; 260-272
dc.identifier.issn
2154-1264
dc.identifier.uri
http://hdl.handle.net/11336/24638
dc.description.abstract
The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WASP, and transcription/splicing factors p54nrb and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Landes Biosciences
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Nuclear Actin
dc.subject
Nuclear Speckles
dc.subject
Co-Transcriptional Splicing
dc.subject
Rna Pol Ii
dc.subject
Transcription/Splicing
dc.subject
Hox Genes
dc.subject.classification
Bioquímica y Biología Molecular
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias Biológicas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-18T14:31:54Z
dc.identifier.eissn
2154-1272
dc.journal.volume
4
dc.journal.number
5
dc.journal.pagination
260-272
dc.journal.pais
Estados Unidos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Philadelphia
dc.description.fil
Fil: Naum Ongania, Gabriela. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Díaz Cortés, Víctor Manuel. Institut Municipal d’Investigació Medica. Barcelona; España
dc.description.fil
Fil: Blasi, Francesco. Istituto FIRC di Oncologia Molecolare. Milan; Italia
dc.description.fil
Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Transcription
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4161/trns.27672
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.4161/trns.27672
Archivos asociados