Datos de investigación
Life history, behavior, physiology, and morphology of Phyllotis vaccarum (Rodentia: Cricetidae) at diverse elevations in the Andes Mountains
Autores:
Patterson, Fiona Alexandra
; Ruperto, Emmanuel Fabián
; Vicenzi, Nadia Pamela
; Menéndez Sammartino, Josefina
; Sassi, Paola Lorena
; Ruperto, Emmanuel Fabián
; Vicenzi, Nadia Pamela
; Menéndez Sammartino, Josefina
; Sassi, Paola Lorena
Publicador:
Consejo Nacional de Investigaciones Científicas y Técnicas
Fecha de depósito:
21/10/2024
Fecha de creado:
2/2011-3/2022
Clasificación temática:
Resumen
This dataset contains life history, behavior, physiology, and morphology traits of Phyllotis vaccarum (Rodentia: Cricetidae) collected at different elevations (1700 m, 2300 m, and 3100 m a.s.l.) in the Andes Mountains of Argentina. Regarding life history data, we present the litter size, the early body mass of pups, and their growth rate. These data derive from females collected during two periods: between February 2011 and March 2015, and between December 2016 and January 2018. Regarding behavioral and physiological data, we show experimental recordings of boldness using an elevated plus maze (EPM), neophobia and exploratory rate using an open field test (OFT), and foraging latency using a radial arm maze (RAM). Additionally, we show measurements of resting metabolic rate using an open-flow respirometry system. These data were collected between November 2021 and March 2022. For morphological data, we present weight, as well as hind foot, tail, head-body, and ear lengths of adult male and female individuals (excluding pregnant ones), obtained during the spring-summer months of each year between 2016 and 2022 (except 2020).
Métodos
All individuals were collected at the study area using Sherman traps (8×9×24 cm, Möller®) baited with oats and peanut butter. Pregnant females were used to obtain life-history traits, while only adult males were utilized to obtain behavioral and physiological data, as female responses may be influenced by their reproductive condition. After capture, individuals were transported to the laboratory, where they were individually housed in cages (30×30×40 cm) equipped with cotton and wood chip bedding and a Polyvinyl Chloride (PVC) shelter (10 cm wide and 20 cm long), under a 12L:12D photoperiod, at 22 ± 2°C, and 25-35% relative humidity (standard laboratory conditions). They were provided with chinchilla food pellets and water ad libitum. The collected females gave birth within 20 days of arriving at the laboratory. After birth, we recorded the litter size (i.e. number of pups), the weight of each pup at 15 days of age as an estimator of early body mass, and the weight of each pup from 15 to 90 days of age to estimate growth rate. At day 30 pups were weaned. Dams and their offspring were maintained under standard laboratory conditions. Boldness was assessed using an elevated plus maze (EPM). This device consisted of an elevated platform with two open and two enclosed arms, each 5 cm wide and 50 cm long, arranged in a plus-sign (+) configuration. The enclosed arms faced each other and had opaque walls and a roof, while the open arms consisted of perpendicular walkways without any walls). The entire structure was placed on a tripod 1 meter above the ground. To begin the test, each animal was removed from its cage and placed at the center of the apparatus. The animal would initially enter one of the enclosed arms. From that point, the time spent in the open arms was recorded over a 60-minute period Neophobia and exploratory rate were assessed using an open field test (OFT). This device consisted of a 1m² arena, devoid of objects, and surrounded by 60 cm high walls to prevent escape. A grid of 100 squares, each measuring 10×10 cm, was drawn on the arena floor. To begin the test, each animal was placed in a corner of the arena within a PVC shelter (6 cm in diameter and 15 cm long, containing cotton and wood chips), allowing the animal to choose when to leave the shelter and voluntarily start exploring. The time taken to leave the shelter was recorded as an indicator of neophobia, and the number of squares crossed in the 5 minutes following shelter exit was recorded as a measure of the exploratory rate. To quantify foraging latency, a radial arm maze (RAM) with 6 arms (55 x 5 cm each) was used, arranged equidistantly around a central platform 10 cm wide. A slice of carrot was placed at the end of one of the arms as bait. During the trials, each individual was placed on the central platform, and the time taken to start eating the bait was recorded. All behavioral recordings were made using a Sony DCR-SR40 video camera. Once the test began, the experiment was conducted without observers present. Given that the study species is nocturnal, all recordings were made during the dark phase of the photoperiod. During the recording period, the room was illuminated with a dim red light to facilitate later viewing of the video recordings. Resting metabolic rate (RMR) was assessed by measuring the rate of oxygen consumption (VO2) in resting, non-fasted animals, within their thermoneutral zone using an open?flow respirometry system, following the methodology described by Menéndez et al. (2022). Briefly, each animal was weighed and placed in a metabolic chamber, where it received a flow of air previously scrubbed of CO2 and H2O. The excurrent air was sampled once per second to measure O2 concentration using an FMS O2 analyzer (Sable Systems). Oxygen consumption was calculated following equation 10.1 from Lighton (2008) using EXPEDATA data acquisition software (Sable Systems). During the 4-hour measurement period, the chamber was maintained at 30°C, i.e. within the thermoneutral zone of the species, using a Peltier-effect cabinet, which maintains constant ambient temperature via a PELT-5 temperature controller (Sable Systems). Initially, metabolism recordings exhibited high fluctuations, likely due to handling disturbances. However, they gradually decreased and stabilized over time. Therefore, we calculated the RMR from the lowest consecutive and most stable 5-minute period within the final 2 hours of recording. The order of behavioral and metabolic measurements for each individual was randomized. Each animal was given a 48-hour rest period between experiments, and all assays were conducted within 10 days of their arrival at the laboratory.
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Datos de Investigación(IADIZA)
Datos de Investigación de INST. ARG DE INVEST. DE LAS ZONAS ARIDAS
Datos de Investigación de INST. ARG DE INVEST. DE LAS ZONAS ARIDAS
Citación
Patterson, Fiona Alexandra; Ruperto, Emmanuel Fabián; Vicenzi, Nadia Pamela; Menéndez Sammartino, Josefina; Sassi, Paola Lorena; (2024): Life history, behavior, physiology, and morphology of Phyllotis vaccarum (Rodentia: Cricetidae) at diverse elevations in the Andes Mountains. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/246178
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