Artículo
The role of galectin-3: From oligodendroglial differentiation and myelination to demyelination and remyelination processes in a cuprizone-induced demyelination model
Hoyos, Hernán Carlos
; Marder, Nora Mariel
; Ulrich, R.; Gudi, V.; Stangel, M.; Rabinovich, Gabriel Adrián
; Pasquini, Laura Andrea
; Pasquini, Juana Maria
Fecha de publicación:
10/2016
Editorial:
Springer International Publishing
Revista:
Advances In Experimental Medicine And Biology.
ISSN:
0065-2598
e-ISSN:
2214-8019
ISBN:
978-3-319-40762-3
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The aim of this work was to combine our previously published results with our new data to show how galectin-3 (Gal-3) controls myelin integrity and function, promotes oligodendroglial cell differentiation, and regulates microglial responses to limit cuprizone- (CPZ)-induced demyelination and foster remyelination. In this study, 8-week-old Gal-3-deficient (Lgals3−/−) and wild type (WT) mice were fed a diet containing 0.2 % CPZ w/w for 6 weeks, after which CPZ was withdrawn in order to allow remyelination. Our results show that remyelination was less efficient in Lgals3−/− than in WT mice. Electron microscopic images from remyelinated sections in Lgals3−/− mice revealed collapsed axons with a defective myelin wrap, while remyelinated WT mice had normal axons without relevant myelin wrap disruption. MMP-3 expression increased during remyelination in WT but not in Lgals3−/− mice. The number of CD45+, TNFa+ and TREM-2b+ cells decreased only in WT mice only, with no alterations in Lgals3−/− mice during demyelination and remyelination. Therefore, Gal-3 influences remyelination by mechanisms involving the tuning of microglial cells, modulation of MMP activity, and changes in myelin architecture.
Palabras clave:
Galectin-3
,
Remyelination
,
Mmps
,
Microglia
,
Oligodendrocytes
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Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Hoyos, Hernán Carlos; Marder, Nora Mariel; Ulrich, R.; Gudi, V.; Stangel, M.; et al.; The role of galectin-3: From oligodendroglial differentiation and myelination to demyelination and remyelination processes in a cuprizone-induced demyelination model; Springer International Publishing; Advances In Experimental Medicine And Biology.; 949; 10-2016; 311-332
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