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dc.contributor.author
Peretti, Leandro Ezequiel
dc.contributor.author
González, Verónica Doris Guadalupe
dc.contributor.author
Marcipar, Iván Sergio
dc.contributor.author
Gugliotta, Luis Marcelino
dc.date.available
2017-09-18T20:03:25Z
dc.date.issued
2017-03
dc.identifier.citation
Peretti, Leandro Ezequiel; González, Verónica Doris Guadalupe; Marcipar, Iván Sergio; Gugliotta, Luis Marcelino; Diagnosis of toxoplasmosis in pregnancy. Evaluation of latex–protein complexes by immnunoagglutination; Cambridge University Press; Parasitology; 144; 8; 3-2017; 1073-1078
dc.identifier.issn
0031-1820
dc.identifier.uri
http://hdl.handle.net/11336/24522
dc.description.abstract
The aim of this work was to obtain a reagent based on latex particles for ruling out acute toxoplasmosis in pregnant women by immunoagglutination (IA). Latex-protein complexes (LPC) were previously synthesized coupling the recombinant protein of Toxoplasma gondii P22Ag and the homogenate of the parasite to latex particles with different size, chemical functionality and charge density. LPC were tested in IA assays against a panel of 72 pregnant women serum samples. Results were analysed through receiver operating characteristic curves, determining area under the curve (AUC), sensitivity, specificity positive and negative predictive values (PPV and NPV, respectively). It was observed that the antigenicity of proteins was not affected during sensitization by either physical adsorption or covalent coupling. The best results in the sense of maximizing discrimination of low avidity sera from chronic ones were observed for the IA test based on latex particles with carboxyl functionality and the recombinant P22Ag, obtaining an AUC of 0·94, a sensitivity of 100% and a NPV of 100%. In this way, the proposed test could be useful for the toxoplasmosis diagnosis in pregnant women, with the advantages of being cheap, rapid and easy to be implemented.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cambridge University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acute Toxoplasmosis
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Acute-Phase Recombinant Antigen
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Immunoagglutination
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Latex–Protein Complexes
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Toxoplasma Gondii
dc.subject.classification
Compuestos
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Ingeniería de los Materiales
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Diagnosis of toxoplasmosis in pregnancy. Evaluation of latex–protein complexes by immnunoagglutination
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-08T14:32:25Z
dc.journal.volume
144
dc.journal.number
8
dc.journal.pagination
1073-1078
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Peretti, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Marcipar, Iván Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.journal.title
Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1017/S0031182017000294
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/diagnosis-of-toxoplasmosis-in-pregnancy-evaluation-of-latexprotein-complexes-by-immnunoagglutination/4F837AE998FF6DB4AF0C34EB5A76B72F
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