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Artículo

The β2-adrenergic agonist salbutamol inhibits migration, invasion and metastasis of the human breast cancer MDA-MB-231 cell line

Rivero, Ezequiel MarianoIcon ; Perez, CeciliaIcon ; Gargiulo, LucíaIcon ; Entschladen, Frank; Zänker, Kurt; Bruzzone, ArianaIcon ; Luthy, Isabel AliciaIcon
Fecha de publicación: 2017
Editorial: Bentham Science Publishers
Revista: Current Cancer Drug Targets
ISSN: 1568-0096
e-ISSN: 1873-5576
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular; Patología

Resumen

Background: Breast cancer is the most diagnosed and the major cause of cancer death in women worldwide. Metastasis is the main cause of these deaths. The metastatic cascade involves multiple steps and it has been described that adrenergic receptors can modulate this process at multiple levels. However, β-adrenergic action in breast cancer is controversial. We have previously shown that β-adrenergic agonists inhibit cell proliferation and tumor growth of numerous breast cancer models. Objective: The purpose of the present investigation was to evaluate adrenergic effect in parameters related to tumor progression (migration, invasion and metastases) in two human breast cancer cell lines. Method: Migration was assessed in IBH-6 and MDA-MB-231 cells by time-lapse videomicroscopy and modified Boyden chambers. Invasion was evaluated by Transwells coated with Matrigel and expression of pro-metastatic genes was determined by RT-qPCR. Experimental metastases studies were performed by injection of the cells in the tail vein of NSG immuno-deficient mice. Results: In both cell lines, salbutamol (β2-agonist) and propranolol (β-blocker) significantly diminished cell migration while epinephrine exerted opposite effects. Moreover, salbutamol inhibited invasion of both breast cancer cell lines and enhanced adhesion to extracellular matrix. Salbutamol treatment was also able to decrease the expression of pro-metastatic genes in MDA-MB-231 cells. Finally, this compound decreased the number and size of MDA-MB-231 lung experimental metastases in NSG immuno- deficient mice. No effect on the establishment of IBH-6 metastases was observed. Conclusion: Our results suggest that salbutamol could be an effective adjuvant drug for the treatment of metastatic breast cancer.
Palabras clave: Breast Cancer , Metastasis , Adrenoceptors , Salbutamol , Mda-Mb-231 , Ibh-6
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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URI: http://hdl.handle.net/11336/24518
URL: http://www.eurekaselect.com/151233/article
DOI: http://dx.doi.org/10.2174/1568009617666170330151415
Colecciones
Articulos(INIBIBB) [351]
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Articulos(IBYME) [1500]
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Rivero, Ezequiel Mariano; Perez, Cecilia; Gargiulo, Lucía; Entschladen, Frank; Zänker, Kurt; et al.; The β2-adrenergic agonist salbutamol inhibits migration, invasion and metastasis of the human breast cancer MDA-MB-231 cell line; Bentham Science Publishers; Current Cancer Drug Targets; 17; 2017; 1-14
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