Evento
Nicotinic Acetylcholine Receptor Clusters Localization On Lipid Domains Of The Plasmalemma
Kamerbeek, Constanza Belén
; Borroni, Maria Virginia
; Pediconi, Maria Filomena
; Barrantes, Francisco Jose
Tipo del evento:
Congreso
Nombre del evento:
47th Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XLVII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Fecha del evento:
30/10/2011
Institución Organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular;
Título de la revista:
Biocell
Editorial:
Instituto de Histología y Embriología “Dr. Mario H. Burgos”
ISSN:
0327-9545
e-ISSN:
1667-5746
Idioma:
Inglés
Clasificación temática:
Resumen
In the present work we have attempted to establish whether there is a correlation between antibody-induced nicotinic acetylcholine receptor (AChR)-rich clusters and the physical state of the underlying cell membrane in living cells. For this purpose di-4- ANEPPDHQ, a fluorescent probe that differentiates liquid-ordered from liquid-disordered phases in model membranes was used in combination with labeling of the AChR in CHO-K1/A5, a clonal cell line expressing adult muscle-type AChR. The so-called generalized polarization (“GP”) of di-4-ANEPPDHQ was measured in regions of the cell-surface membrane associated with the AChR platforms clearly identified using conventional widefield fluorescence microscopy. Under control conditions AChR clusters are roughly equally distributed among liquid-ordered and liquid-disordered domains. This distribution changes upon cyclodextrin-mediated cholesterol depletion or by Latrunculin disruption of the actin cytoskeleton. Association of AChR clusters with lipid domains with different biophysical properties may have consequences on AChR trafficking processe.
Palabras clave:
ACETYLCHOLINE NICOTINIC RECEPTOR
,
LIPID DOMAINS
,
DI-4-ANEPPDHQ
,
CHOLESTEROL
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Eventos(INIBIBB)
Eventos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Eventos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Citación
Nicotinic Acetylcholine Receptor Clusters Localization On Lipid Domains Of The Plasmalemma; 47th Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XLVII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes San Luis; Argentina; 2011; 71-71
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