Mostrar el registro sencillo del ítem
dc.contributor.author
Fernandez Miyakawa, Mariano Enrique
dc.contributor.author
Fisher, Derek J.
dc.contributor.author
Poon, Rachael
dc.contributor.author
Sayeed, Sameera
dc.contributor.author
Adams, Vicki
dc.contributor.author
Rood, Julian I.
dc.contributor.author
McClane, Bruce A.
dc.contributor.author
Uzal, Francisco A.
dc.date.available
2024-09-23T13:03:29Z
dc.date.issued
2007-03
dc.identifier.citation
Fernandez Miyakawa, Mariano Enrique; Fisher, Derek J.; Poon, Rachael; Sayeed, Sameera; Adams, Vicki; et al.; Both Epsilon-Toxin and Beta-Toxin Are Important for the Lethal Properties of Clostridium perfringens Type B Isolates in the Mouse Intravenous Injection Model; American Society for Microbiology; Infection and Immunity; 75; 3; 3-2007; 1443-1452
dc.identifier.issn
0019-9567
dc.identifier.uri
http://hdl.handle.net/11336/244810
dc.description.abstract
Clostridium perfringens is capable of producing up to 15 toxins, including alpha-toxin (CPA), beta-toxin (CPB), epsilon-toxin (ETX), enterotoxin, beta2-toxin (CPB2), and perfringolysin O. Type B isolates, which must produce CPA, CPB, and ETX, are associated with animal illnesses characterized by sudden death or acute neurological signs, with or without intestinal damage. Type B pathogenesis in ruminants is poorly understood, with some animals showing lesions and clinical signs similar to those caused by either type C or type D infections. It is unknown whether host or environmental conditions are dominant for determining the outcome of type B disease or if disease outcomes are determined by variable characteristics of type B isolates. To help clarify this issue, 19 type B isolates were evaluated for toxin production during late-log-phase growth via quantitative Western blotting and by biological activity assays. Most type B isolates produced CPB levels similar to those produced by type C isolates in vitro and have the potential to produce genotype C-like disease. The lethality of type B isolate supernatants administered intravenously to mice was evaluated with or without prior trypsin treatment, and monoclonal antibody neutralization studies also were performed. Correlation analyses comparing toxin levels in type B supernatants versus lethality and neutralization studies both found that the main contributor to lethality without pretreatment with trypsin was CPB, whereas neutralization studies indicated that CPB and ETX were both important after trypsin pretreatment. At least part of the CPB produced by type B isolates remained active after trypsin treatment. However, the overall lethalities of most supernatants were lower after trypsin pretreatment. Also, there was a significant association between ETX, CPB2, and CPA production in vitro among type B isolates. However, our results suggest that both CPB and ETX are likely the most important contributors to the pathogenesis of C. perfringens type B infections in domestic animals.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
EPSILON-TOXIN
dc.subject
BETA-TOXIN
dc.subject
SUDDEN DEATH
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Both Epsilon-Toxin and Beta-Toxin Are Important for the Lethal Properties of Clostridium perfringens Type B Isolates in the Mouse Intravenous Injection Model
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-09-19T13:48:17Z
dc.journal.volume
75
dc.journal.number
3
dc.journal.pagination
1443-1452
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Davis; Estados Unidos
dc.description.fil
Fil: Fisher, Derek J.. University of Pittsburgh; Estados Unidos
dc.description.fil
Fil: Poon, Rachael. Monash University; Australia
dc.description.fil
Fil: Sayeed, Sameera. Monash University; Australia
dc.description.fil
Fil: Adams, Vicki. Monash University; Australia
dc.description.fil
Fil: Rood, Julian I.. Monash University; Australia
dc.description.fil
Fil: McClane, Bruce A.. Monash University; Australia. University of Pittsburgh; Estados Unidos
dc.description.fil
Fil: Uzal, Francisco A.. University of California at Davis; Estados Unidos
dc.journal.title
Infection and Immunity
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/iai.01672-06
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/iai.01672-06
Archivos asociados