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dc.contributor.author Matos, María Laura
dc.contributor.author Lapyckyj, Lara
dc.contributor.author Rosso, Marina
dc.contributor.author Besso, María José
dc.contributor.author Mencucci, Maria Victoria
dc.contributor.author Marin Briggiler, Clara Isabel
dc.contributor.author Giustina, Silvina
dc.contributor.author Furlong, Laura Ines
dc.contributor.author Vazquez, Monica Hebe
dc.date.available 2017-09-18T18:57:38Z
dc.date.issued 2016-12
dc.identifier.citation Matos, María Laura; Lapyckyj, Lara; Rosso, Marina; Besso, María José; Mencucci, Maria Victoria; et al.; Identification of a novel human E-Cadherin splice variant andassessment of its effects upon EMT-related events; Wiley; Journal of Cellular Physiology; 232; 6; 12-2016; 1368-1386
dc.identifier.issn 0021-9541
dc.identifier.uri http://hdl.handle.net/11336/24478
dc.description.abstract Epithelial Cadherin (E-cadherin) is involved in calcium-dependent cell-cell adhesion and signal transduction. The E-cadherin decrease/loss is a hallmark of Epithelial to Mesenchymal Transition (EMT), a key event in tumor progression. The underlying molecular mechanisms that trigger E-cadherin loss and consequent EMT have not been completely elucidated. This study reports the identification of a novel human E-cadherin variant mRNA produced by alternative splicing. A bioinformatics evaluation of the novel mRNA sequence and biochemical verifications suggest its regulation by Nonsense-Mediated mRNA Decay (NMD). The novel E-cadherin variant was detected in 29/42 (69%) human tumor cell lines, expressed at variable levels (E-cadherin variant expression relative to the wild type mRNA = 0.05-11.6%). Stable transfection of the novel E-cadherin variant in MCF-7 cells (MCF7Ecadvar) resulted in downregulation of wild type E-cadherin expression (transcript/protein) and EMT-related changes, among them acquisition of a fibroblastic-like cell phenotype, increased expression of Twist, Snail, Zeb1, and Slug transcriptional repressors and decreased expression of ESRP1 and ESRP2 RNA binding proteins. Moreover, loss of cytokeratins and gain of vimentin, N-cadherin and Dysadherin/FXYD5 proteins was observed. Dramatic changes in cell behavior were found in MCF7Ecadvar, as judged by the decreased cell-cell adhesion (Hanging-drop assay), increased cell motility (Wound Healing) and increased cell migration (Transwell) and invasion (Transwell w/Matrigel). Some changes were found in MCF-7 cells incubated with culture medium supplemented with conditioned medium from HEK-293 cells transfected with the E-cadherin variant mRNA. Further characterization of the novel E-cadherin variant will help understanding the molecular basis of tumor progression and improve cancer diagnosis.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley
dc.rights info:eu-repo/semantics/embargoedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CANCER
dc.subject EPITHELIAL CADHERIN
dc.subject EPITHELIAL TO MESENCHYMAL TRANSITION
dc.subject ALTERNATIVE SPLICING
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.subject.classification Patología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Identification of a novel human E-Cadherin splice variant andassessment of its effects upon EMT-related events
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-09-08T20:19:16Z
dc.identifier.eissn 1097-4652
dc.journal.volume 232
dc.journal.number 6
dc.journal.pagination 1368-1386
dc.journal.pais Estados Unidos
dc.journal.ciudad Hoboken
dc.conicet.avisoEditorial Esta es la versión revisada por pares del siguiente artículo Matos, María Laura; Lapyckyj, Lara; Rosso, Marina; Besso, María José; Mencucci, Maria Victoria; et al.; Identification of a Novel Human E-Cadherin Splice Variant and Assessment of its Effects upon EMT-Related Events; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Physiology; 232; 6; 6-2017; 1368-1386 que se ha publicado en forma final en http://dx.doi.org/10.1002/jcp.25622 Este artículo puede ser utilizado con fines no comerciales de acuerdo con Wiley Terms y condiciones para el auto-archivado.
dc.description.fil Fil: Matos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Lapyckyj, Lara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Rosso, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Besso, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Mencucci, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Giustina, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Furlong, Laura Ines. Universitat Pompeu Fabra; España
dc.description.fil Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.journal.title Journal of Cellular Physiology
dc.rights.embargoDate 2018-01-01
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jcp.25622
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.25622/abstract


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info:eu-repo/semantics/embargoedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)