Artículo
Activation of retinoid X receptors protects retinal neurons and pigment epithelial cells from BMAA-induced death
Soto, Tamara Belen
; Tenconi, Paula Estefania
; Buzzi, Edgardo David
; Dionisio, Leonardo Raul
; Mateos, Melina Valeria
; Rotstein, Nora Patricia
; Spitzmaul, Guillermo Federico
; Politi, Luis Enrique
; German, Olga Lorena
Fecha de publicación:
26/08/2024
Editorial:
Elsevier Science
Revista:
Biochimica et Biophysica Acta-Molecular Cell Research
ISSN:
0167-4889
e-ISSN:
1879-2596
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Exposure to the non-protein amino acid cyanotoxin β–N-methylamino-L-alanine (BMAA), released by cyanobacteria found in many water reservoirs has been associated with neurodegenerative diseases. We previously demonstrated that BMAA induced cell death in both retina photoreceptors (PHRs) and amacrine neurons by triggering different molecular pathways, as activation of NMDA receptors and formation of carbamate-adducts was only observed in amacrine cell death. We established that activation of Retinoid X Receptors (RXR) protects retinal cells, including retina pigment epithelial (RPE) cells from oxidative stress-induced apoptosis. We now investigated the mechanisms underlying BMAA toxicity in these cells and those involved in RXR protection. BMAA addition to rat retinal neurons during early development in vitro increased reactive oxygen species (ROS) generation and polyADP ribose polymers (PAR) formation, while pre-treatment with serine (Ser) before BMAA addition decreased PHR death. Notably, RXR activation with the HX630 agonist prevented BMAA-induced death in both neuronal types, reducing ROS generation, preserving mitochondrial potential, and decreasing TUNEL-positive cells and PAR formation. This suggests that BMAA promoted PHR death by substituting Ser in polypeptide chains and by inducing polyADP ribose polymerase activation. BMAA induced cell death in ARPE-19 cells, a human epithelial cell line; RXR activation prevented this death, decreasing ROS generation and caspase 3/7 activity. These findings suggest that RXR activation prevents BMAA harmful effects on retinal neurons and RPE cells, supporting this activation as a broad-spectrum strategy for treating retina degenerations
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Articulos(INIBIBB)
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Articulos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Citación
Soto, Tamara Belen; Tenconi, Paula Estefania; Buzzi, Edgardo David; Dionisio, Leonardo Raul; Mateos, Melina Valeria; et al.; Activation of retinoid X receptors protects retinal neurons and pigment epithelial cells from BMAA-induced death; Elsevier Science; Biochimica et Biophysica Acta-Molecular Cell Research; 1871; 8; 26-8-2024; 1-14
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