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dc.contributor.author
González, Norma Viviana
dc.contributor.author
Barbeito, Claudio Gustavo
dc.date.available
2024-09-20T11:07:05Z
dc.date.issued
2011-10
dc.identifier.citation
González, Norma Viviana; Barbeito, Claudio Gustavo; Colchicine-induced apoptosis daily rhythms in male and female suckling mice; Nova Science Publishers; Animal Biology Journal; 2; 1; 10-2011; 11-20
dc.identifier.issn
1949-498X
dc.identifier.uri
http://hdl.handle.net/11336/244692
dc.description.abstract
Cell death in the intestinal crypts is increased in many conditions including exposure to several cytotoxic compounds, among them colchicine. In despite of in vitro studies´ abundance, in vivo investigations assessing the effect of this microtubule inhibitor on apoptosis are scarce and no data dealing specifically with colchicine-induced apoptosis in suckling mice are available. The aims were to determine the colchicine inductive effect on apoptosis in 14 d old mice intestinal crypts; to establish the existence of daily variations in the apoptotic indices and to examine whether there is sexual dimorphism in such variations. Experimental work was performed on C3H/S male and female 14 d old mice exposed to 12:12 light-dark conditions. Commencing at midnight and every 4 h thereafter animals were sacrificed having received an i.p. 1 µg colchicine dose per g body weight 4 h before. Samples of duodenum and colon were processed for hematoxylineosin staining and TUNEL technique. Twenty longitudinally sectioned crypts per organ and individual were monitored for apoptotic cells. The apoptotic index was expressed as the number of apoptotic cells per 1,000 nuclei for each animal. The arithmetic mean ± SE was calculated for each study time and gender. Differences between genders were determined by the Student’s t test. Within group differences were tested by one-way analysis of variance (ANOVA). Results revealed that. 1) the apoptotic daily changes phasing in the small bowel between young males and females showed sexual dimorphism; 2) the absence of daily variations in the young female large bowel cell death indices; 3) a highly prominent induced apoptosis intensity in the small bowel; 4) the sensitivity and number of colchicine induced-apoptosis changes throughout the day are age-dependant. Colchicine is to be taken regularly as a treatment for various human diseases and poisoning results in a multi-organ dysfunction syndrome, particularly affecting tissues with rapid cell turnover among them the intestinal epithelium. The complex pattern of variables implied in the colchicine-induced intestinal apoptosis rhythms (gender, age, time of drug delivery) highlights the need of treatments design with colchicine dosing according with the patient condition.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nova Science Publishers
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Apoptosis
dc.subject
Colchicine
dc.subject
Circadian rhythms
dc.subject
Intestinal crypts
dc.subject
Age
dc.subject
Gender
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Colchicine-induced apoptosis daily rhythms in male and female suckling mice
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-08-21T13:05:17Z
dc.journal.volume
2
dc.journal.number
1
dc.journal.pagination
11-20
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: González, Norma Viviana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
dc.description.fil
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
dc.journal.title
Animal Biology Journal
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