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Artículo

Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons

Cenzano, Ana MaríaIcon ; Cantoro, RenataIcon ; Hernández Sotomayor, S. M. Teresa; Abdala, Guillermina IreneIcon ; Racagni, Graciela Esther
Fecha de publicación: 05/12/2011
Editorial: American Chemical Society
Revista: Journal of Agricultural and Food Chemistry
ISSN: 0021-8561
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid (PA); digalactosyldiacylglycerol (DGDG) was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidylethanolamine (PE). The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate (DGPP) by phosphatidic acid kinase (PAK). This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation.
Palabras clave: Solanum Tuberosum , Potato , Electrospray Ionization Tandem Mass Spectrometry , Phospholipid , Galactolipid , Kinase
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/24408
URL: http://pubs.acs.org/doi/abs/10.1021/jf204269y
DOI: http://dx.doi.org//10.1021/jf204269y
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580764/
Colecciones
Articulos(CCT-CENPAT)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CENPAT
Articulos(IFEVA)
Articulos de INST.D/INV.FISIOLOGICAS Y ECO.VINCULADAS A L/AGRIC
Citación
Cenzano, Ana María; Cantoro, Renata; Hernández Sotomayor, S. M. Teresa; Abdala, Guillermina Irene; Racagni, Graciela Esther; Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons; American Chemical Society; Journal of Agricultural and Food Chemistry; 60; 1; 5-12-2011; 418-426
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