Artículo
Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
Cenzano, Ana María
; Cantoro, Renata
; Hernández Sotomayor, S. M. Teresa; Abdala, Guillermina Irene
; Racagni, Graciela Esther
Fecha de publicación:
05/12/2011
Editorial:
American Chemical Society
Revista:
Journal of Agricultural and Food Chemistry
ISSN:
0021-8561
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid (PA); digalactosyldiacylglycerol (DGDG) was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidylethanolamine (PE). The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate (DGPP) by phosphatidic acid kinase (PAK). This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT-CENPAT)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CENPAT
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CENPAT
Articulos(IFEVA)
Articulos de INST.D/INV.FISIOLOGICAS Y ECO.VINCULADAS A L/AGRIC
Articulos de INST.D/INV.FISIOLOGICAS Y ECO.VINCULADAS A L/AGRIC
Citación
Cenzano, Ana María; Cantoro, Renata; Hernández Sotomayor, S. M. Teresa; Abdala, Guillermina Irene; Racagni, Graciela Esther; Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons; American Chemical Society; Journal of Agricultural and Food Chemistry; 60; 1; 5-12-2011; 418-426
Compartir
Altmétricas