Mostrar el registro sencillo del ítem
dc.contributor.author
Cerliani, María Belén
dc.contributor.author
Richard, Silvina Mariel
dc.date.available
2017-09-15T17:05:29Z
dc.date.issued
2013-11
dc.identifier.citation
Cerliani, María Belén; Richard, Silvina Mariel; Polymorphism Analysis of Genes Involved in Xenobiotic Metabolism and Circadian Rhythm in Human Breast Cancer; Science and Engineering Publishing Company; International Journal of Advance in Medical Science (AMS); 1; 4; 11-2013; 57-64
dc.identifier.issn
2327-7238
dc.identifier.uri
http://hdl.handle.net/11336/24386
dc.description.abstract
Individual response to xenobiotic exposures depends on the dynamics of xenobiotic metabolism and the circadian clock system, among other factors. Since these systems are closely related, polymorphisms in their key genes may have an impact on how carcinogenic compounds are metabolized, and therefore on the risk of tumor development. Whereas the mammary gland is exposed to agents that damage DNA, it was considered of high interest to study xenobiotic metabolizing genes (XMG) and clock genes in this tissue. Our aim was to analyze genotype and allele frequencies of polymorphisms in the XMG N-acetyl transferase 2 (NAT2) and glutathione S-transferase T1 (GSTT1), and in the clock genes period 3 (PER3) and CLOCK in human breast tumor samples. As well, it was if these polymorphisms were in linkage disequilibrium. 65 samples were genotyped for polymorphisms in GSTT1 (null), NAT2 (C481T, G590A and G857A), CLOCK (T3111C) and PER3 (length polymorphism), by PCR and PCR-RFLP. For GSTT1, 20% of the samples showed total absence of the gene. When NAT2 genotypes were grouped by their associated acetylator phenotype, 5% of rapid, 49% of intermediate and 46% of slow acetylator phenotypes were indicated. Allele frequencies for CLOCK were T=0.78 and C=0.22; for PER3, they were 0.66 for the 4-repeats allele and 0.34 for the 5-repeats allele. Linkage disequilibrium test indicated evidence of strong linkage between NAT2 and CLOCK (χ2=13.076; p=0.005). With regard to allele and genotype frequencies, our results are in agreement with those reported for similar populations. The evidence of linkage disequilibrium in our breast cancer samples is interesting and requires further investigation. This work constitutes a first approximation to a combined study of polymorphisms in XMG and clock genes in breast cancer Argentinian patients. Future studies will attempt to address the role of these and other polymorphisms in cancer risk, prognosis and response to treatment.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Science and Engineering Publishing Company
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Breast Cancer
dc.subject
Xenobiotic Metabolism
dc.subject
Circadian Rhythm
dc.subject
Nat2
dc.subject
Gstt1
dc.subject
Clock Genes
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Polymorphism Analysis of Genes Involved in Xenobiotic Metabolism and Circadian Rhythm in Human Breast Cancer
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-14T13:56:23Z
dc.identifier.eissn
2327-7629
dc.journal.volume
1
dc.journal.number
4
dc.journal.pagination
57-64
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Indiana
dc.description.fil
Fil: Cerliani, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
dc.description.fil
Fil: Richard, Silvina Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
dc.journal.title
International Journal of Advance in Medical Science (AMS)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.seipub.org/ams/paperInfo.aspx?ID=3722#Abstract
Archivos asociados