Biosynthesis of anti-HCV compounds using extremophile microorganisms

This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2?-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2?-deoxyriboside. The obtained compounds can be used as antiviral agents.

Palabras clave: GEOBACILLUS STEAROTHERMOPHILUS , NUCLEOSIDE ANALOGUES , GREEN CHEMISTRY , ENTRAPMENT IMMOBILIZATION

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

Identificadores

URI: http://hdl.handle.net/11336/243820

URL: http://www.sciencedirect.com/science/article/pii/S0960894X12010451?v=s5

DOI: http://dx.doi.org/10.1016/j.bmcl.2012.08.045

Colecciones

Articulos(CINDECA)
Articulos de CENTRO DE INV EN CS.APLICADAS "DR.JORGE J.RONCO"

Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL

Citación

Rivero, Cintia Wanda; de Benedetti, Eliana Celeste; Sambeth, Jorge Enrique; Lozano, Mario Enrique; Trelles, Jorge Abel; Biosynthesis of anti-HCV compounds using extremophile microorganisms; Pergamon-Elsevier Science Ltd; Biorganic and Medicinal Chemistry Letters; 22; 19; 10-2012; 6059-6062

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