Artículo
An experimental, hands‐on approach to epithelial ion transport: A simple technique for introducing students to ion transport in epithelia
Bagdadi, Andrea; Orona, Nadia Soledad
; Fernández, Eugenio; Altamirano, Anibal Alberto
; Amorena, Carlos Ernesto
Fecha de publicación:
09/2010
Editorial:
John Wiley & Sons
Revista:
Biochemistry and Molecular Biology Education
ISSN:
1470-8175
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We have realized that our Biology undergraduate students learn biological concepts as established truths without awareness of the body of experimental evidence supporting the emerging models as usually presented in handbooks and texts in general. Therefore, we have implemented a laboratory practice in our course of Physiology and Biophysics, aimed to introduce the students in the way the scientific models and theories are built, through the measurement of Na+ transport in frog skin. Transepithelial Na+ transport was assessed in the frog skin, with measurements of short circuit currents. The mucosal Na+ and serosal K+ concentrations were modified and the effects were recorded. These effects were reversible. Addition of a drug that blocks epithelial Naþ channels (amiloride) to the mucosal side solution abolished the short circuit current. Sodium fluxes were calculated, and the results were adjusted to Michaelis-Menten kinetics. The impact of the proposed practice on the students is discussed.
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Bagdadi, Andrea; Orona, Nadia Soledad; Fernández, Eugenio; Altamirano, Anibal Alberto; Amorena, Carlos Ernesto; An experimental, hands‐on approach to epithelial ion transport: A simple technique for introducing students to ion transport in epithelia; John Wiley & Sons; Biochemistry and Molecular Biology Education; 38; 5; 9-2010; 303-308
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